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Figure S1. m157 expression and phenotype of NK cells in WT, m157-Tg, B2m-/- and m157-Tg B2m-/- mice. (A) Representative dot plots demonstrating H-2Kb/H-2Db and m157 expression on peripheral blood lymphocytes from WT, m157-Tg, β2m-/- and m157-Tg b2m-/- mice. (B) Representative dot plots demonstrating CD69 and CD11b expression on freshly isolated splenic NK cells from WT, m157-Tg, β2m-/- and m157-Tgb2m-/- mice. The numbers represent the percentage of Ly49H+ or Ly49H- NK cells expressing CD69 or CD11b. The dot plots were gated on NK cells (NK1.1+, CD3- cells).

Figure S2. Cytokines combinations can partially reverse activating receptor-mediated NK cell hyporesponsiveness. The ratio of the percentage of IFN-γ producing Ly49H+ NK cells to the percentage of IFN-γ producing Ly49H- NK cells (A) with and without TNF-α and IL-12 (n= 4 for WT without cytokines, n= 4 for WT with cytokines, n= 5 for m157-Tg without cytokines, n= 5 for m157-Tg with cytokines). (B) with and without TNF-α and IFN-β (n= 4 for WT without cytokines, n= 4 for WT with cytokines, n= 5 for m157-Tg without cytokines, n= 5 for m157-Tg with cytokines). (C) with and without TNF- α, IL-12 and IFN-β (n= 6 for WT without cytokines, n= 6 for WT with cytokines, n= 9 for m157-Tg without cytokines, n= 9 for m157-Tg with cytokines). The results are presented as the mean ± SEM of the indicated number of mice pooled from two separate experiments. **p < 0.05 (Student's t-test).

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