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Vol 41 (12) 2011, DOI: 10.1002/eji.201141631

Similar inflammatory DC maturation signatures induced by TNF or Trypanosoma brucei antigens instruct default Th2-cell responses

Katrien Pletinckx, Benoit Stijlemans, Vladimir Pavlovic, Regina Laube, Carolin Brandl, Susanne Kneitz, lain Beschin, Patrick De Baetselier and Manfred B. Lutz.

Unfortunately, there is an error in Supporting Information Fig. 5B and C in the above article because the cells were not gated on CD4+ and Vβ5+ cells as indicated in the legend. When the cells are gated accordingly, there is an increase in absolute numbers of CD4+ Vβ5+ CD25+ FoxP3+ cells following injection of DCs matured with either TNF-α, mfVSG, MiTat1.5 sVSG or LPS and loaded with OVA peptide, as shown in the corrected Supporting Information Fig. 5C below (left panel); however, the increase is similar regardless of maturation stimulus, although the increase is only statistically significant for TNF-α (*p<0.05, one-way ANOVA followed by Bonferroni post-testing). The increase reflects the overall increased cellularity and size (data not shown) of DC-immunized spleens in comparison with naive animals. Accordingly, when the percentages of CD4+ Vβ5+ CD25+ FoxP3+ cells in the spleen are calculated, no such increases are observed (corrected Supporting Information Fig. 5C below, right panel). The analysed data shown in the right panel of corrected Supporting Information Fig. 5C replace the representative plots shown in the original Supporting Information Fig. 5B.

Finally, the following text on page p. 3485:

“Furthermore, injection of DC conditioned with TNF, mfVSG, or MiTat1.5 sVSG did not raise the frequency or total cellular amounts of FoxP3+ Treg cells among OVA-TCR-specific T cells in vivo similar to LPS-matured DCs (Supporting Information Fig. 5B and C) further strengthening the observation that partially mature DCs efficiently induce proliferation and priming of (CFSE-labeled) OVA-TCR-specific T cells in vivo (Fig. 4A).”

is amended to read:

“Furthermore, injection of DC conditioned with TNF, mfVSG, or MiTat1.5 sVSG did not raise the frequency or total cellular amounts of FoxP3+ Treg cells among OVA-TCR-specific T cells in vivo in comparison with injection of LPS-matured DCs (Supporting Information Fig. 5B and C) further strengthening the observation that partially mature DCs efficiently induce proliferation and priming of (CFSE-labeled) OVA-TCR-specific T cells in vivo (Fig. 4A).”

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Supplementary Figure 5C. Mice received CD4+ CD25−OT-II T cells intravenously followed by a single injection of DC matured with the indicated stimuli and loaded with OVA peptide. Induction of FoxP3* Treg cells was analysed 6 days post-injection by flow cytometry. Absolute cell numbers (left panel) and percentages (right panel) of CD4* Vβ5* CD25* FoxP3* cells. Data are mean+SD of 3 individual mice per condition ((*p<0.05, one-way ANOVA followed by Bonferroni post-testing).