An evolutionarily conserved mutual interdependence between Aire and microRNAs in promiscuous gene expression
Article first published online: 17 MAY 2013
© 2013 The Authors. European Journal of Immunology published byWiley-VCH Verlag GmbH & Co. KGaA Weinheim.
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European Journal of Immunology
Volume 43, Issue 7, pages 1769–1778, July 2013
How to Cite
Ucar, O., Tykocinski, L.-O., Dooley, J., Liston, A. and Kyewski, B. (2013), An evolutionarily conserved mutual interdependence between Aire and microRNAs in promiscuous gene expression. Eur. J. Immunol., 43: 1769–1778. doi: 10.1002/eji.201343343
- Issue published online: 4 JUL 2013
- Article first published online: 17 MAY 2013
- Accepted manuscript online: 16 APR 2013 03:23AM EST
- Manuscript Accepted: 11 APR 2013
- Manuscript Revised: 12 MAR 2013
- Manuscript Received: 15 JAN 2013
- Helmholtz Postdoc Program
- Fonds Wetenschappelijk Onderzoek
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Figure S1. Gating strategy used for TEC sorting. (A) Mouse TEC-enriched cell suspensions were sorted as follows: PI-negative, CD45pos cells were sorted as thymocytes. PI-negative, CD45neg cells were separated into EpCAMposLy51high (cTECs) and EpCAMposLy51low (mTECs). mTECs were further separated into MHClow and MHChigh. (B) Human TEC-enriched cell suspensions were sorted as follows: DAPI-negative, CD45pos cells were sorted as thymocytes. DAPI-negative, CD45neg cells were separated into EpCAMposCDR2high (cTECs) and EpCAMposCDR2low (mTECs). mTECs were further separated into MHClow and MHChigh. (C) Mouse TEC-enriched cell suspensions derived from Adig mice were sorted as follows: PI-negative, CD45pos cells were sorted as thymocytes. PI-negative, CD45negEpCAMpos cells were separated into CD80lowGFPlow (CD80low), CD80highGFPlow (CD80highAireneg) and CD80highGFPhigh (CD80highAirepos).
Figure S2. Progressive structural disorganisation of Dicerdeficient mutant thymi. Thymic sections obtained from 4-, 10- and 14-week old Dicer null mutants and wildtype littermate controls were stained for cytokeratin-14, cytokeratin-8 and Aire. Epithelial voids are indicated with white asterisks. Note that the medulla disorganisation progressed with age, yet Airepos cells were still numerous in the Dicer mutants at 10 weeks. At 14 weeks of age, the Dicer null mutant thymus was very small and only a few cytokeratin-14-positive cells were detected. Aire staining is therefore not included for this age.
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