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Table S1. Overview of the effects of MO- and PMN-MDSCs on various aspects of CD8+ T-cell activation.

Table S2. List of commercial antibodies used for flow cytometry

Figure S1. MO- and PMN-MDSCs were purified from the spleens of EG7-OVA tumor-bearing WT, IFN-γR-/-, STAT-1-/- and IRF-1-/- mice.

Figure S2. MO- and PMN-MDSCs differentially depend on IFN-γ, STAT-1 and IRF-1 to activate their anti-proliferative capacity.

Figure S3. MO-MDSCs respond more heterogeneously to an IFN-γ challenge than PMN-MDSCs, as measured by STAT-1 phosphorylation.

Figure S4. Gating strategy on CD8+ OT-1 T cells after 24 h and 42 h culture.

Figure S5. PMN-MDSCs increase IFN-γ secretion levels upon co-culture with OVA-stimulated OT-1 splenocytes.

Figure S6. IFN-γR-/- and IRF-1-/- MDSCs enhance IFN-γ production by activated CD8+ T cells on a per cell basis.

Figure S7. MO- and PMN-MDSCs do not augment IL-12 levels upon co-culture with OVA-stimulated OT-1 splenocytes.

Figure S8. MO- and especially PMN-MDSCs suppress T-bet expression in activated CD8+ T cells.

Figure S9. MDSCs down-modulate IL-2 production by activated CD8+ T cells.

Figure S10. MO-MDSCs down-regulate CD25 expression and STAT5 phosphorylation.

Figure S11. MDSCs alter the expression levels of cell adhesion molecules on CD8+ T cells.

Figure S12. MO-MDSCs augment Fas expression on activated CD8+ T cells.

Figure S13. Neither MO- nor PMN-MDSCs are targets for OVA-specific CTLs, nor do they affect the cytotoxic activity of mature CTLs.

Figure S14. Unseparated splenic MDSCs affect CD8+ T-cell activation events.

Figure S15. RMA-OVA-induced splenic MDSCs affect CD8+ T-cell activation events.

Figure S16. MDSCs differentially affect CD8+ T-cell activation events upon polyclonal stimulation.

Figure S17. Tumor-infiltrating MO-MDSCs are strongly anti-proliferative and recapitulate only some aspects of their splenic counterparts.

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