SEARCH

SEARCH BY CITATION

As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.

FilenameFormatSizeDescription
eji2757-sup-0001-Suppmat.doc2640K

Figure S1. Purity of cells upon magnetic cell sorting and FACS sorting Splenic PDC were first magnetically enriched. Then, the Sca-1+ and Sca-1- PDC subpopulation was further isolated according to the characteristic expression of mPDCA-1 and Sca-1 using FACS sorting with purities > 99%. Shown is data representative of > 3 experiments.

Figure S2. Coexpression of Ly49Q, CCR9, Sca-1 and CX3CR1 on murine PDC (A) Single cell suspensions from spleen and bone marrow (BM) of BALB/c mice were stained with antibodies directed against mPDCA-1, Ly49Q, CCR9 and Sca-1. Gated mPDCA-1+ PDC were analyzed for a coexpression of Ly49Q, CCR9 and Sca-1. (B) Siglec-H+ PDC from BM of untreated Cx3cr1gfp/+ mice were analyzed for a coexpression of CX3CR1, Sca-1, CCR9 and Ly49Q. Shown is data representative of 2–3 experiments.

Figure S3. Sca-1 expression on cells from BM, spleen and LN (A) Single cell suspensions from BM, spleen and LN of BALB/c mice were stained with antibodies directed against Siglec-H and Sca-1. Shown is data representative of 5 independent experiments.

Table S1. Agilent reporters with an at least two-fold upregulation in Sca1+ cells relative to Sca1- cells (t-test p-value ≤ 0.05)

Table S2. Agilent reporters with an at least two-fold downregulation in Sca1+ cells relative to Sca1- cells (t-test p-value ≤ 0.05)

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.