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eji2737-sup-0001-FigureS1.pdf23KFigure S1. Myelin-reactive CXCR3-/- Th1 cells readily infiltrate the CNS of adoptive transfer recipients. Draining LN cells from MOG35–55/ CFA immunized WT (left) or CXCR3-/- (right) mice were cultured with antigen under Th1- polarizing conditions prior to transfer into CD45.1 congenic hosts. Spinal cord mononuclear cells from each cohort (n = 5 mice/ group) were pooled at the end of the experiment and analyzed by flow cytometry to quantify the absolute total number of CD45.2+ donor cells and CD45.1+ congenic host cells per spinal cord. The experiment was repeated twice with similar results.

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