Emergence of a distinct HIV-specific IL-10-producing CD8+ T-cell subset with immunomodulatory functions during chronic HIV-1 infection
Article first published online: 29 AUG 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 43, Issue 11, pages 2875–2885, November 2013
How to Cite
Clutton, G., Yang, H., Hancock, G., Sande, N., Holloway, C., Angus, B., von Delft, A., Barnes, E., Borrow, P., Pellegrino, P., Williams, I., McMichael, A. and Dorrell, L. (2013), Emergence of a distinct HIV-specific IL-10-producing CD8+ T-cell subset with immunomodulatory functions during chronic HIV-1 infection. Eur. J. Immunol., 43: 2875–2885. doi: 10.1002/eji.201343646
- Issue published online: 20 NOV 2013
- Article first published online: 29 AUG 2013
- Accepted manuscript online: 30 JUL 2013 02:17AM EST
- Manuscript Accepted: 26 JUL 2013
- Manuscript Revised: 17 JUN 2013
- Manuscript Received: 23 APR 2013
- Oxford NIHR Biomedical Research Centre
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Supplementary Figure 1 Progressive gating strategy used to identify CD4+ T cells, CD8+ T cells and CD19+ B cells within the lymphocyte population and CD3- CD14+ cells within the monocyte population.
Supplementary Figure 2 Frequencies of CD4+ T cells, CD8+ T cells, CD19+ B cells and CD14+ monocytes that constitutively express IL-10 among ART-naive patients (n=25), ART-treated patients (n=20) and uninfected controls (n=5).
Supplementary Figure 3 Effect of depletion of HIV-1 gag-specific IL-10+ CD8+ T cells on HIV-1 gag-induced expression of HLA-DR and CD38 on CD4+ T cells.
Supplementary Figure 4 (A) CD38 expression on CD14+ monocytes from infected (p24 Ag+) and mock-infected PBMC (n = 4) after 3 and 5 days’ culture. CD8+ T cell-depleted PBMC from four HIV-negative subjects were activated for 3 days with phytohaemagglutinin and then infected with HIV-1BaL in the presence of IL-2, using a MOI to achieve infection of 5-10% CD4+ T cells, as indicated by expression of p24 antigen (p24 Ag). (B) Representative plots showing p24 Ag expression in monocytes from mock-infected PBMC cultures (left) and HIV-1BaL-infected PBMC cultures (right) after 3 days. (C) CD38 expression on CD14+ monocytes in mock-infected PBMC cultures (unexposed uninfected, UU), uninfected (p24 Agneg) monocytes in HIV-1BaL-infected PBMC (exposed uninfected, EU), or infected (p24 Ag+) monocytes in HIV-1BaL-infected PBMC (exposed infected, EI) (n = 6). Median values are indicated by horizontal bars.
Supplementary Figure 5 CD38 expression by monocytes in cultures where all CD8+ T cells were present (Undepleted), IL-10+ CD8+ T cells were depleted prior to co-culture of CD8+ and CD8neg fractions (“Depleted”) and where the CD8neg fraction was incubated with an IL-10R-blocking antibody prior to co-culture with undepleted CD8+ T cells (“Undepleted + αIL-10R”). Mean fluorescence intensity is expressed as arbitrary units. Three donors were tested; median values are indicated by horizontal bars.
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