Get access

Artificial [FeFe]-Hydrogenase: On Resin Modification of an Amino Acid to Anchor a Hexacarbonyldiiron Cluster in a Peptide Framework

Authors

  • Souvik Roy,

    1. Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287-1604, USA, Fax: +1-4809652747
    2. Center for Bioenergy and Photosynthesis, Arizona State University, Tempe, AZ 85287-1604, USA
    Search for more papers by this author
  • Sandip Shinde,

    1. Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287-1604, USA, Fax: +1-4809652747
    2. Center for Bioenergy and Photosynthesis, Arizona State University, Tempe, AZ 85287-1604, USA
    Search for more papers by this author
  • G. Alexander Hamilton,

    1. Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287-1604, USA, Fax: +1-4809652747
    Search for more papers by this author
  • Hilairy E. Hartnett,

    1. Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287-1604, USA, Fax: +1-4809652747
    2. Center for Bioenergy and Photosynthesis, Arizona State University, Tempe, AZ 85287-1604, USA
    3. School of Earth and Space Exploration, Arizona State University, Tempe, AZ 85287-1404, USA
    Search for more papers by this author
  • Anne K. Jones

    1. Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287-1604, USA, Fax: +1-4809652747
    2. Center for Bioenergy and Photosynthesis, Arizona State University, Tempe, AZ 85287-1604, USA
    Search for more papers by this author

Abstract

A general method for immobilization of synthetic analogues of the [FeFe]-hydrogenase in designed peptides via on resin modification of an amino acid side chain with a dithiol functional group is described. Utilizing a unique amine side chain as anchor, the dithiol unit is coupled to the peptide via formation of an amide. This dithiol unit precisely positions the two required sulfur atoms for the formation of a [(μ-SRS){Fe(CO)3}2] cluster on reaction with [Fe3(CO)12]. UV/Vis and FTIR spectroscopy demonstrate formation of the desired complex.

Ancillary