Further Characterization of the [FeFe]-Hydrogenase Maturase HydG

Authors

  • Cécile Tron,

    1. Laboratoire de Chimie et Biologie des Métaux, Université Joseph Fourier, UMR 5249-CNRS, iRTSV CEA Grenoble, 17, Avenue des Martyrs, 38054 Grenoble Cedex, France
    2. Laboratoire de Cristallographie et de Cristallogenèse des Protéines, Institut de Biologie Structurale J.-P. Ebel. CEA, CNRS, Université Joseph Fourier, 41, Jules Horowitz, 38027 Grenoble Cedex 1, France, Fax: +33-4-38785122
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  • Mickaël V. Cherrier,

    1. Laboratoire de Cristallographie et de Cristallogenèse des Protéines, Institut de Biologie Structurale J.-P. Ebel. CEA, CNRS, Université Joseph Fourier, 41, Jules Horowitz, 38027 Grenoble Cedex 1, France, Fax: +33-4-38785122
    2. BM16-CRG Consorci Laboratori de Llum de Sincrotró (LLS) c/o ESRF, 38043 Grenoble, France
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  • Patricia Amara,

    1. Laboratoire de Cristallographie et de Cristallogenèse des Protéines, Institut de Biologie Structurale J.-P. Ebel. CEA, CNRS, Université Joseph Fourier, 41, Jules Horowitz, 38027 Grenoble Cedex 1, France, Fax: +33-4-38785122
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  • Lydie Martin,

    1. Laboratoire de Cristallographie et de Cristallogenèse des Protéines, Institut de Biologie Structurale J.-P. Ebel. CEA, CNRS, Université Joseph Fourier, 41, Jules Horowitz, 38027 Grenoble Cedex 1, France, Fax: +33-4-38785122
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  • Francois Fauth,

    1. BM16-CRG Consorci Laboratori de Llum de Sincrotró (LLS) c/o ESRF, 38043 Grenoble, France
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  • Edmundo Fraga,

    1. BM16-CRG Consorci Laboratori de Llum de Sincrotró (LLS) c/o ESRF, 38043 Grenoble, France
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  • Mélanie Correard,

    1. Laboratoire de Cristallographie et de Cristallogenèse des Protéines, Institut de Biologie Structurale J.-P. Ebel. CEA, CNRS, Université Joseph Fourier, 41, Jules Horowitz, 38027 Grenoble Cedex 1, France, Fax: +33-4-38785122
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  • Marc Fontecave,

    1. Laboratoire de Chimie et Biologie des Métaux, Université Joseph Fourier, UMR 5249-CNRS, iRTSV CEA Grenoble, 17, Avenue des Martyrs, 38054 Grenoble Cedex, France
    2. Collège de France, 11, Place Marcelin-Berthelot, 75005 Paris, France
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  • Yvain Nicolet,

    1. Laboratoire de Cristallographie et de Cristallogenèse des Protéines, Institut de Biologie Structurale J.-P. Ebel. CEA, CNRS, Université Joseph Fourier, 41, Jules Horowitz, 38027 Grenoble Cedex 1, France, Fax: +33-4-38785122
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  • Juan C. Fontecilla-Camps

    1. Laboratoire de Cristallographie et de Cristallogenèse des Protéines, Institut de Biologie Structurale J.-P. Ebel. CEA, CNRS, Université Joseph Fourier, 41, Jules Horowitz, 38027 Grenoble Cedex 1, France, Fax: +33-4-38785122
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Abstract

Recent work conducted in several laboratories has shown that the [FeFe]-hydrogenase maturase HydG synthesizes both CO and CN from tyrosine. We have very recently found that although CN synthesis does not need the [4Fe-4S] cluster found in the HydG C-terminal domain, CO synthesis does require it. We have also proposed that the +H2N·–CH2–CO2 radical is a precursor for these two active site ligands. Here, we have extended our characterization of both the wild-type enzyme and a ThiH-like HydG truncated mutant, with small angle X-ray scattering (SAXS) spectroscopy, homology modeling and functional studies.

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