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Keywords:

  • Medicinal chemistry;
  • Ferrocene;
  • Vanadium;
  • DNA cleavage;
  • Photodynamic therapy

Abstract

Ferrocene-conjugated oxidovanadium(IV) complexes [VO(Fc-tpy)(B)](ClO4)2 (14) and [VO(Ph-tpy)(dppz)](ClO4)2 (5) as a control [Fc = (η5-C5H4)FeII5-C5H5), Fc-tpy = 4′-ferrocenyl-2,2′:6′,2″-terpyridine, Ph-tpy = 4′-phenyl-2,2′:6′,2″-terpyridine, B = heterocyclic base: 2,2′-bipyridine (bpy in 1), 1,10-phenanthroline (phen in 2), dipyridoquinoxaline (dpq in 3), dipyridophenazine (dppz in 4)] were prepared and their DNA binding, DNA photocleavage activity and photocytotoxicity studied. The crystal structure of [VO(Fc-tpy)(bpy)](PF6)2·3Me2CO shows a vanadyl group in six-coordinate VIVON5 coordination geometry, in which Fc-tpy and bpy display tridentate meridional and bidentate N-donor axial–equatorial binding modes, respectively. The one-electron paramagnetic complexes exhibit a charge-transfer band near 590 nm in DMF. The VIV/VIII redox couple in 14 appears near –0.7 V, whereas the Fc moiety shows a response near 0.6 V vs. SCE in DMF/0.1 M TBAP. The complexes are good binders to calf thymus DNA with Kb values of 104–106M–1. DNA melting and viscometric data suggest groove and/or partial intercalative DNA binding of the complexes. Complexes 35 display DNA photocleavage activity in near-IR light of 785 nm. Complex 4 shows significant photocytotoxicity in visible light (400–700 nm) in HeLa cells with low dark toxicity.