Further Characterization of the [FeFe]-Hydrogenase Maturase HydG (pages 1121–1127)
Cécile Tron, Mickaël V. Cherrier, Patricia Amara, Lydie Martin, Francois Fauth, Edmundo Fraga, Mélanie Correard, Marc Fontecave, Yvain Nicolet and Juan C. Fontecilla-Camps
Article first published online: 2 FEB 2011 | DOI: 10.1002/ejic.201001101
We have explored the CO/CN– synthesis catalyzed by [FeFe]-hydrogenase maturase HydG with site-directed mutants, and have performed functional studies, SAXS spectroscopy and homology modeling. We conclude that whereas CN– is made in the active site-containing TIM-barrel domain, the CO precursor follows an internal path from this site to a catalytic [4Fe-4S] cluster in the tightly bound C-terminal domain.