In this study, the tocopherol (T) and tocotrienol (T3) contents of grape seeds from 14 different varieties grown in Korea were analyzed using saponification extraction followed by normal-phase liquid chromatography. α-T, γ-T, α-T3, and γ-T3 were detected in all samples. The total concentration of tocopherol and tocotrienol was in the range of 4.8–9.9 mg/100 g seed (35.3–68.8 mg/100 g oil basis). The Muscat Bailey A cultivar had the highest total tocopherol and tocotrienol contents, followed by Canner and Naples. γ-T3 ranged from 1.6 to 4.9 mg/100 g seed (11.2 to 53.81 mg/100 g oil basis) and was the main isomer, followed by α-T3 in most of the samples. Analytical method validation parameters including accuracy and precision were determined. Overall recovery from grape seeds was close to 100%.