Tocopherols and tocotrienols in grape seeds from 14 cultivars grown in Korea



In this study, the tocopherol (T) and tocotrienol (T3) contents of grape seeds from 14 different varieties grown in Korea were analyzed using saponification extraction followed by normal-phase liquid chromatography. α-T, γ-T, α-T3, and γ-T3 were detected in all samples. The total concentration of tocopherol and tocotrienol was in the range of 4.8–9.9 mg/100 g seed (35.3–68.8 mg/100 g oil basis). The Muscat Bailey A cultivar had the highest total tocopherol and tocotrienol contents, followed by Canner and Naples. γ-T3 ranged from 1.6 to 4.9 mg/100 g seed (11.2 to 53.81 mg/100 g oil basis) and was the main isomer, followed by α-T3 in most of the samples. Analytical method validation parameters including accuracy and precision were determined. Overall recovery from grape seeds was close to 100%.