Research Article

A comparison of the indirect and direct quantification of glycidol ester by kinetic analysis

  1. Masao Shimizu1,*,
  2. Naoto Kudo1,
  3. Hiroki Shiro2,
  4. Koichi Yasunaga3,
  5. Yoshinori Masukawa2,
  6. Yoshihisa Katsuragi1,
  7. Takeshi Yasumasu1

Article first published online: 30 JUN 2011

DOI: 10.1002/ejlt.201000517

Issue

European Journal of Lipid Science and Technology

European Journal of Lipid Science and Technology

Volume 113, Issue 8, pages 985–991, August 2011

Additional Information

How to Cite

Shimizu, M., Kudo, N., Shiro, H., Yasunaga, K., Masukawa, Y., Katsuragi, Y. and Yasumasu, T. (2011), A comparison of the indirect and direct quantification of glycidol ester by kinetic analysis. Eur. J. Lipid Sci. Technol., 113: 985–991. doi: 10.1002/ejlt.201000517

Author Information

  1. 1

    Healthcare Research Laboratories, Kao Corporation, 2-1-3 Bunka, Sumida-ku, Tokyo, Japan

  2. 2

    Analytical Science Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai-machi, Haga-gun, Tochigi, Japan

  3. 3

    Kao Health and Nutrition LLC, 500 Park Blvd, Itasca, IL, USA

*Healthcare Food Research Lab., Kao Corporation, 2-1-3 Bunka, Sumida-ku, Tokyo 131-8501, Japan Fax: +81-3-5630-7457.

Publication History

  1. Issue published online: 15 AUG 2011
  2. Article first published online: 30 JUN 2011
  3. Accepted manuscript online: 7 JUN 2011 04:30AM EST
  4. Manuscript Accepted: 18 APR 2011
  5. Manuscript Revised: 20 MAR 2011
  6. Manuscript Received: 29 OCT 2010

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Keywords:

  • Direct method;
  • Indirect method;
  • Glycidol fatty acid esters;
  • Kinetic analysis;
  • Quantification

Abstract

In a comparison of the German society's official indirect method and the direct LC-MS method to determine the levels of glycidol fatty acid esters (GEs) in edible oils, the indirect method showed lower GE levels in cases of a high level of GEs and/or containing partial acylglycerols (PGs). The present study used kinetic analysis to compare the scope of both methods. A kinetic model combination of reversible decomposition of GEs and 3-monochloro-1,2-propanediol forming substances (MCPD-FS) generated from PGs accurately predicted a persistent level of underestimation of commercial vegetable oils when the indirect method was used. The results of the kinetic prediction showed that the underestimation in the indirect method was proportional to the PG and GE levels in oils. Although most conventional cooking oils are low in GEs and PGs, significant error may occur in oils such as palm oil, which are reported to have a higher content of GEs and DAGs. The direct method was affected by neither the GEs nor the PGs, and proved to be a truer and more accurate determinant of GE levels in a wide range of edible oil products.

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