Enzymatic preparation of L-α-glycerylphosphorylcholine in an aqueous medium

Authors

  • Kangyi Zhang,

    1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, P. R. China
    2. Institute of Agricultural Products Processing, Henan Academy of Agriculture Sciences, Zhengzhou, Henan Province, P. R. China
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  • Yuanfa Liu,

    1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, P. R. China
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  • Xingguo Wang

    Corresponding author
    1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, P. R. China
    • State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu Province, P. R. China Fax: 086-510-85876799
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Abstract

L-α-Glycerylphosphorylcholine (L-α-GPC) was successfully prepared from phosphatidylcholine (PC) of food-grade soy lecithin powder using a novel enzymatic reaction in an aqueous medium. 94.5% yield of L-α-GPC was obtained under the optimal conditions of 55°C, 6.67 mg/mL substrate, 2 mM CaCl2, and 33.4 U/mL phospholipase A1 (Lecitase Ultra). L-α-GPC at 98% purity, 73.4% (wt%) recovery, and specific rotation (equation image) of −2.5° was achieved by silica gel column chromatography. Owing to its excellent catalytic efficiency, low cost, and ready availability, phospholipase A1 (Lecitase Ultra) provides a very satisfactory option for converting PC to L-α-GPC.

Practical applications: L-α-Glycerylphosphorylcholine (L-α-GPC) has been studied recently for its potential use as a supplement that may support neurological functions, but it is only found in trace amounts in nature. The present results indicate that Lecitase Ultra can be used for producing L-α-GPC from aqueous PC and suggest encouraging prospects for practical or industrial applications utilizing its notable catalytic performance, economy, and convenience.

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