Structured phosphatidylcholine was successfully produced by immobilized phospholipase A1 catalyzed acidolysis of phosphatidylcholine and CLA. Response surface methodology was applied to optimize the reaction system using three process parameters: enzyme load, temperature, and substrates molar ratio. Optimal conditions obtained from the model were 15% enzyme load at 55°C for a 1:4 (PC/CLA) substrates molar ratio, which produced a yield of 85.8% of CLA incorporation. The total correlation coefficient (R2) was 0.92 and no lack of fit was detected. This suggests the fitness of the model obtained and it suggests that the model is sufficiently accurate to estimate the incorporation of CLA into PC.
Practical applications: This study could contribute to process development for enzyme catalyzed phospholipid modification. By changing the fatty acid composition of lecithin, delivery of desired (beneficial) fatty acids could be better achieved. Additionally, emulsification properties of the phospholipids could open a wide area of applications in the food and cosmetic industries.