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Figure S1. Degenerated oligonucleotide primers utilized for RT-PCR amplification of the EpLPAT2 gene. Conserved motifs for different A-class LPAT enzymes that were used in the design of the oligonucleotides are also shown.


Figure S2. Predicted transmembrane domains and topology for the Echium LPAT. (A) TMpred (, (B) TMHMM ( software tools were used to predict transmembrane domains (marked in red) for EpLPAT2. (C) TOPO2 ( software was used to generate a two-dimensional topology model. AT motifs are indicated.


Table S1. Fatty acid composition (% of total fatty acids) in diverse organs of Echium pitardii

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