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FilenameFormatSizeDescription
ejlt201300195-sm-0001-SuppFig-S1.doc22K

Figure S1. Degenerated oligonucleotide primers utilized for RT-PCR amplification of the EpLPAT2 gene. Conserved motifs for different A-class LPAT enzymes that were used in the design of the oligonucleotides are also shown.

ejlt201300195-sm-0002-SuppFig-S2.docx334K

Figure S2. Predicted transmembrane domains and topology for the Echium LPAT. (A) TMpred (http://www.ch.embnet.org/software/TMPRED_form.html), (B) TMHMM (http://www.cbs.dtu.dk/services/TMHMM/) software tools were used to predict transmembrane domains (marked in red) for EpLPAT2. (C) TOPO2 (http://www.sacs.ucsf.edu/TOPO2/) software was used to generate a two-dimensional topology model. AT motifs are indicated.

ejlt201300195-sm-0003-SuppTab-S1.doc29K

Table S1. Fatty acid composition (% of total fatty acids) in diverse organs of Echium pitardii

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