Current address: Grupo de Bioenergía, Centro de Tecnología de REPSOL, Móstoles, Madrid, Spain.
Cloning and molecular characterization of a class A lysophosphatidate acyltransferase gene (EpLPAT2) from Echium (Boraginaceae)
Article first published online: 17 SEP 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Lipid Science and Technology
Special Issue: EuroFedLipid Highlights 2013
Volume 115, Issue 11, pages 1334–1346, November 2013
How to Cite
Mañas-Fernández, A., Arroyo-Caro, J. M., Alonso, D. L. and García-Maroto, F. (2013), Cloning and molecular characterization of a class A lysophosphatidate acyltransferase gene (EpLPAT2) from Echium (Boraginaceae). Eur. J. Lipid Sci. Technol., 115: 1334–1346. doi: 10.1002/ejlt.201300195
- Issue published online: 8 NOV 2013
- Article first published online: 17 SEP 2013
- Accepted manuscript online: 26 AUG 2013 04:28AM EST
- Manuscript Accepted: 18 AUG 2013
- Manuscript Revised: 30 JUL 2013
- Manuscript Received: 5 JUN 2013
- Junta de Andalucia (P05-189-AGR and P08-AGR-3962)
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Figure S1. Degenerated oligonucleotide primers utilized for RT-PCR amplification of the EpLPAT2 gene. Conserved motifs for different A-class LPAT enzymes that were used in the design of the oligonucleotides are also shown.
Figure S2. Predicted transmembrane domains and topology for the Echium LPAT. (A) TMpred (http://www.ch.embnet.org/software/TMPRED_form.html), (B) TMHMM (http://www.cbs.dtu.dk/services/TMHMM/) software tools were used to predict transmembrane domains (marked in red) for EpLPAT2. (C) TOPO2 (http://www.sacs.ucsf.edu/TOPO2/) software was used to generate a two-dimensional topology model. AT motifs are indicated.
Table S1. Fatty acid composition (% of total fatty acids) in diverse organs of Echium pitardii
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