These authors contributed equally to this work.
Matrix-assisted laser desorption/ionization mass spectrometry and Raman spectroscopy: An interesting complementary approach for lipid detection in biological tissues
Article first published online: 15 JUL 2014
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Lipid Science and Technology
Volume 116, Issue 8, pages 1080–1086, August 2014
How to Cite
Jadoul, L., Malherbe, C., Calligaris, D., Longuespée, R., Gilbert, B., Eppe, G. and De Pauw, E. (2014), Matrix-assisted laser desorption/ionization mass spectrometry and Raman spectroscopy: An interesting complementary approach for lipid detection in biological tissues. Eur. J. Lipid Sci. Technol., 116: 1080–1086. doi: 10.1002/ejlt.201300198
- Issue published online: 4 AUG 2014
- Article first published online: 15 JUL 2014
- Accepted manuscript online: 10 JUL 2014 02:03AM EST
- Manuscript Accepted: 10 APR 2014
- Manuscript Revised: 21 MAR 2014
- Manuscript Received: 14 OCT 2013
- ULg Research Council
- European funds for regional development (FEDER)
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Figure S1. Average intensities of (a) [PE (18:0/18:1)+H]+ and (b) [PC (18:0/18:1)+H]+ calculated from six replicate spots, containing a pure GP, measured by MALDI MS using the CHCA, the 2,5-DHB and the 1,5-DAN. Each lipid species has been deposited at the same concentration using the same conditions.
Figure S2. Molecular images of [PC (18:0/18:1)+H]+, [PC (18:0/18:1)+Na]+, [PC (18:0/18:1)+K]+ ionic species measured by MALDI MSI from triplicate homogenate biological sections using the 2,5-DHB and the 1,5-DAN.
Figure S3. Raman spectra of (a) the PC (18:0/18:1), (b) the PC (16:0/14:0), (c) the PE (18:0/18:1), and (d) the PE (16:0/18:1). An intensity scale expansion has been performed on the spectral region below 1000 cm−1.
Figure S4. Overlay of Raman spectra of (a) homogenate biological section; (b) homogenate biological section where the lipids have been extracted; (c) PC (18:0/18:1).
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