New yeast isolate Pichia caribbica synthesizes xylolipid biosurfactant with enhanced functionality

Authors

  • Kasturi Joshi-Navare,

    1. Biochemical Sciences Division, National Chemical Laboratory, Pune, Maharashtra, India
    Search for more papers by this author
  • Pradeep Kumar Singh,

    1. Biochemical Sciences Division, National Chemical Laboratory, Pune, Maharashtra, India
    2. Physical, Material Chemistry Division, National Chemical Laboratory, Pune, Maharashtra, India
    Search for more papers by this author
  • Asmita A. Prabhune

    Corresponding author
    1. Biochemical Sciences Division, National Chemical Laboratory, Pune, Maharashtra, India
    • Correspondence: Dr. Asmita A. Prabhune, Biochemical Sciences Division, National Chemical Laboratory, Homi Bhabha Road, Pune 411008, Maharashtra, India

      E-mail: aa.prabhune@ncl.res.in

      Fax: +91 020 25902648

    Search for more papers by this author

Abstract

Sophorolipids (SL) belong to a class of glycolipidic biosurfactants suitable for a wide range of applications. The structural diversity in SL gives rise to variation in physicochemical and biological properties. To achieve the less explored head group diversity in sophorolipid structure, a new xylose fermenting yeast Pichia caribbica has been employed for biosurfactant production. The media and fermentation parameters have been optimized to achieve maximum yield of 7.48 g/L. The physicochemical properties of the xylolipid biosurfactant have been assessed. It reduced the surface tension of distilled water from 70 to 35.9 mN/m with the low critical micellar concentration (CMC) 1.0 mg/L as compared to typical SL (reported CMC range 40–100 mg/L). Structural characterization was done using FTIR and HR-MS to identify the structure. 17-L-[(β-D-xylopyranosyl)-oxy]-Δ9-heptadecanoic acid correlated to m/z 415 majorly constituted the product. Control experiment was performed in which glucose was provided as the hydrophilic carbon. This product was also subjected to HR-MS analysis to determine its chemical nature and found to be different from xylolipid. Presence of xylose as head group was anticipated to give altered physicochemical and biological activities and indeed a low CMC value and better inhibitory action was demonstrated against Staphylococcus aureus.

Practical applications: Sophorolipids produced by microbial sources have several advantageous properties over commercial petroleum-based surfactants including biodegradability, environmentally friendly nature, and low toxicity. Here, we have attempted to modify the hydrophilic head group by incorporating xylose in place of glucose. This molecule will also behave differently in response to different stimuli. Stimuli-responsive surfactants are a class of compounds with applications in stabilization of emulsions, suspensions or foams, drug encapsulation and delivery, hard-surface cleaning, personal care applications.

ejlt201300363-gra-0001

New yeast isolate – Pichia caribbica was provided with xylose and oleic acid. This resulted in synthesis of glycolipidic molecule different from typical sophorolipid synthesized by Candida bombicola. Xylose was incorporated as head group, i.e., hydrophilic moiety in place of glucose. The variation in structure imparted better surface tension lowering activity thus showed low CMC value. Also antibacterial activity was enhanced.

Ancillary