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Fig. S1. SIM setting Chromatographic separation of a mixture of 14 FAME standards (5 µg/mL), in (a) scan mode, and (b) SIM mode.

Fig. S2. The effect of reaction time on the fatty acids in fish oil capsule (n = 3).

Fig. S3. Chromatographic separation of cod liver oil (after derivatization) by non-polar column, all identified fatty acids were performed by comparison of their mass spectrum profiles and retention time with the synthetic standards.

Fig. S4. Effect of injection port temperature on signal response for 7 FAMEs (1 µg/mL), n = 3. GC conditions: splitless injection (1 µL injected), column temperature program: initial temperature 50°C (2 min), then 50°C/min to 180°C, then by 3°C/min up to 220°C (1 min) and then 15°C/min to 270°C (5 min), and measured in scan mode 50–500 m/z. (The injection temperature 245°C was selected.)

Fig. S5. Effect of carrier gas flow rate on signal response for 7 FAMEs (1 µg/mL, n = 3). The gas flow rate of 1.0 mL/min was selected.

Table S1. FAME standards used in this study

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