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Keywords:

  • DHA;
  • EPA;
  • HPLC;
  • Iodolactonization;
  • Marine oils;
  • n-3 PUFA;
  • Urea complexation

Eicosapentaenoic (EPA, 20:5 n-3) and docosahexaenoic (DHA, 22:6 n-3) acids were extracted from processing wastes of the liver of the squid Berryteuthis magister. The process included three steps: concentration of the total polyunsaturated fatty acids (PUFA) with urea; separation of individual FA by iodolactonization method, and obtainment of highly purified EPA and DHA by preparative HPLC. Concentration of the acids with urea was carried out at a ratio of urea/FA 3:1 at temperatures of 0 and −20°C, this allowed us to raise the concentration of EPA and DHA from 7.8 and 6.5% to 32.5 and 32.0%, respectively. The iodolactonization reaction was used to obtain from the concentrate individual preparations with concentrations of 93.4% for EPA and 95.6% for DHA; the total yields of the original FA were 50.7 and 41.7%, respectively. Differences in the rates of formation of γ- and δ-iodolactones made a sequential extraction possible, first of DHA from the total PUFA, then of EPA from the remaining acids. Purification of the obtained PUFA products to more than 99% purity was conducted by preparative HPLC.

Practical applications: The proposed three-step procedure for separating EPA and DHA is designed to produce n-3 PUFA concentrates and highly purified EPA and DHA. The obtained products can be recommended for scientific experiments in vitro.

ejlt201400083-gra-0001

The proposed three-step procedure for separating EPA and DHA is designed to produce n-3 PUFA concentrates and highly purified EPA and DHA.