Concentration and purification of polyunsaturated fatty acids from squid liver processing wastes
Article first published online: 15 JUL 2014
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Lipid Science and Technology
Volume 116, Issue 11, pages 1608–1613, November 2014
How to Cite
Latyshev, N. A., Ermolenko, E. V. and Kasyanov, S. P. (2014), Concentration and purification of polyunsaturated fatty acids from squid liver processing wastes. Eur. J. Lipid Sci. Technol., 116: 1608–1613. doi: 10.1002/ejlt.201400083
- Issue published online: 5 NOV 2014
- Article first published online: 15 JUL 2014
- Accepted manuscript online: 23 JUN 2014 01:59AM EST
- Manuscript Accepted: 17 JUN 2014
- Manuscript Revised: 2 JUN 2014
- Manuscript Received: 12 FEB 2014
- Marine oils;
- n-3 PUFA;
- Urea complexation
Eicosapentaenoic (EPA, 20:5 n-3) and docosahexaenoic (DHA, 22:6 n-3) acids were extracted from processing wastes of the liver of the squid Berryteuthis magister. The process included three steps: concentration of the total polyunsaturated fatty acids (PUFA) with urea; separation of individual FA by iodolactonization method, and obtainment of highly purified EPA and DHA by preparative HPLC. Concentration of the acids with urea was carried out at a ratio of urea/FA 3:1 at temperatures of 0 and −20°C, this allowed us to raise the concentration of EPA and DHA from 7.8 and 6.5% to 32.5 and 32.0%, respectively. The iodolactonization reaction was used to obtain from the concentrate individual preparations with concentrations of 93.4% for EPA and 95.6% for DHA; the total yields of the original FA were 50.7 and 41.7%, respectively. Differences in the rates of formation of γ- and δ-iodolactones made a sequential extraction possible, first of DHA from the total PUFA, then of EPA from the remaining acids. Purification of the obtained PUFA products to more than 99% purity was conducted by preparative HPLC.
Practical applications: The proposed three-step procedure for separating EPA and DHA is designed to produce n-3 PUFA concentrates and highly purified EPA and DHA. The obtained products can be recommended for scientific experiments in vitro.
The proposed three-step procedure for separating EPA and DHA is designed to produce n-3 PUFA concentrates and highly purified EPA and DHA.