These authors contributed equally to this work.
Non-esterified fatty acids induce apoptosis via a ROS-dependent mechanism involving the mitochondrial pathway in bovine abomasal smooth muscle cells
Version of Record online: 8 JUL 2014
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Lipid Science and Technology
Volume 116, Issue 11, pages 1477–1484, November 2014
How to Cite
Wei, T., Tian, W. and Xie, G. (2014), Non-esterified fatty acids induce apoptosis via a ROS-dependent mechanism involving the mitochondrial pathway in bovine abomasal smooth muscle cells. Eur. J. Lipid Sci. Technol., 116: 1477–1484. doi: 10.1002/ejlt.201400137
- Issue online: 5 NOV 2014
- Version of Record online: 8 JUL 2014
- Accepted manuscript online: 12 JUN 2014 03:56AM EST
- Manuscript Accepted: 5 JUN 2014
- Manuscript Revised: 20 MAY 2014
- Manuscript Received: 18 MAR 2014
- Natural Science Foundation of China. Grant Number: 31101865
- Abomasal displacement;
- Gastrointestinal disorders;
- Non-esterified fatty acid;
- Oxidative stress
Increased blood non-esterified fatty acid (NEFA) concentrations might be implicated as a factor that plays a role in the pathogenesis of gastrointestinal motility disorder, such as abomasal displacement. We sought to elucidate possible effects of high concentrations of NEFA on primary cultured bovine abomasal smooth muscle cells (BSMC). BSMC treated with high concentrations of NEFA resulted in decrease of cell viability and increase of cell apoptosis rate. NEFA treatment induced oxidative stress, as revealed by increased levels of ROS and MDA, while decreased the antioxidative abilities (SOD, CAT, GSH, and GSSG) in BSMC. Meanwhile, high concentrations of NEFA induced BSMC mitochondrial apoptotic cell death. Mitochondrial membrane potential decrease, increased Bax and decreased Bcl-2, cytochrome c release, caspases activation, and apoptosis inducing factor (AIF) translocation were observed in NEFA-treated cells. Pretreatment with the antioxidant N-acetylcysteine (NAC) effectively reversed these NEFA-induced events. In conclusion, these results show that NEFA induce BSMC apoptosis mainly via a ROS-dependent mechanism involving the mitochondrial pathway.
The cytotoxicity of non-esterified fatty acid (consist of oleic acid, linoleic acid, palmitic acid, stearic acid, and palmitoleic acid) on primary cultured bovine abomasal smooth muscle cell, and the underlying molecular mechanism.