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Keywords:

  • Abomasal displacement;
  • Apoptosis;
  • Gastrointestinal disorders;
  • Non-esterified fatty acid;
  • Oxidative stress

Increased blood non-esterified fatty acid (NEFA) concentrations might be implicated as a factor that plays a role in the pathogenesis of gastrointestinal motility disorder, such as abomasal displacement. We sought to elucidate possible effects of high concentrations of NEFA on primary cultured bovine abomasal smooth muscle cells (BSMC). BSMC treated with high concentrations of NEFA resulted in decrease of cell viability and increase of cell apoptosis rate. NEFA treatment induced oxidative stress, as revealed by increased levels of ROS and MDA, while decreased the antioxidative abilities (SOD, CAT, GSH, and GSSG) in BSMC. Meanwhile, high concentrations of NEFA induced BSMC mitochondrial apoptotic cell death. Mitochondrial membrane potential decrease, increased Bax and decreased Bcl-2, cytochrome c release, caspases activation, and apoptosis inducing factor (AIF) translocation were observed in NEFA-treated cells. Pretreatment with the antioxidant N-acetylcysteine (NAC) effectively reversed these NEFA-induced events. In conclusion, these results show that NEFA induce BSMC apoptosis mainly via a ROS-dependent mechanism involving the mitochondrial pathway.