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Full Paper
Solution-Phase Synthesis of Phosphorothioate Oligonucleotides Using a Solid-Supported Acyl Chloride with H-Phosphonate Chemistry
Article first published online: 31 OCT 2005
DOI: 10.1002/ejoc.200500547
Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Adamo, I., Dueymes, C., Schönberger, A., Navarro, A.-E., Meyer, A., Lange, M., Imbach, J.-L., Link, F., Morvan, F. and Vasseur, J.-J. (2006), Solution-Phase Synthesis of Phosphorothioate Oligonucleotides Using a Solid-Supported Acyl Chloride with H-Phosphonate Chemistry. Eur. J. Org. Chem., 2006: 436–448. doi: 10.1002/ejoc.200500547
Publication History
- Issue published online: 2 JAN 2006
- Article first published online: 31 OCT 2005
- Manuscript Received: 20 JUL 2005
- Abstract
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Keywords:
- Oligonucleotides;
- H-Phosphonate;
- Solution-phase synthesis;
- Solid-supported reagents;
- Nucleosides
Abstract
The synthesis of oligonucleotides from dimers to a hexamer by a H-phosphonate approach in solution using solid-supported acyl chloride is reported herein. This strategy avoids the use of chromatography. The work-ups have been simplified and involve filtration, aqueous extraction and precipitation. The synthesis was scaled up to 1 mmol for the dimers and 340 μmol for the hexamer. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)