Feature Article

Detection of DNA hybridization using self-assembled bilayer lipid membranes (BLMs)

  1. Christina G. Siontorou1,
  2. Dimitrios P. Nikolelis1,*,
  3. Paul A. E. Piunno2,
  4. Ulrich J. Krull2

Article first published online: 25 APR 2005

DOI: 10.1002/elan.1140091407

Issue

Electroanalysis

Electroanalysis

Volume 9, Issue 14, pages 1067–1071, October 1997

Additional Information

How to Cite

Siontorou, C. G., Nikolelis, D. P., Piunno, P. A. E. and Krull, U. J. (1997), Detection of DNA hybridization using self-assembled bilayer lipid membranes (BLMs). Electroanalysis, 9: 1067–1071. doi: 10.1002/elan.1140091407

Author Information

  1. 1

    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, G-15771-Athens, Greece

  2. 2

    Chemical Sensors Group, Department of Chemistry, Erindale Campus, University of Toronto, 3359 Mississauga Road North, Mississauga, Ontario L5L 1C6, Canada

*Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, G-15771-Athens, Greece

Publication History

  1. Issue published online: 25 APR 2005
  2. Article first published online: 25 APR 2005
  3. Manuscript Accepted: 18 FEB 1997
  4. Manuscript Received: 16 JAN 1997

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (600K)

Keywords:

  • Electrochemistry;
  • Biosensor;
  • Self-assembled bilayer lipid membranes;
  • Nucleic acid hybridization;
  • Transduction

Abstract

The present work reports the use of self assembled bilayer lipid membranes supported on the surface of a metal (s-BLMs) as transducers for the direct electrochemical monitoring of DNA hybridization. Characterized oligomers based on single stranded deoxyribonucleic acid (ssDNA) thymidylic acid icosanucleotides that were terminated with C16 (dT20-C16) and deoxyadenylic acid icosanucleotides (dA20) were used for the hybridization procedure at the lipid membrane surface. The decrease of the ion conductivity observed during the hybridization procedure is indicative of the formation of the duplex, which may subsequently be excluded from the membrane. The incorporation of nucleic acids into the lipid matrix was investigated by differential scanning calorimetric (DSC) experiments. The present simple electrochemical biosensor for monitoring of DNA hybridization was regenerable for multiple cycles of application. This approach provides low detection limits for DNA (a few hundreds of fmol), fast response times (on the order of a few minutes), mild conditions of hybridization and capability of analyzing small volumes of sample.

Get PDF (600K)