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Keywords:

  • Biosensors;
  • Clay;
  • Glucose oxidase;
  • Inorganic gel;
  • Mesoporous TiO2 electrode;
  • Polypyrrole

Abstract

A novel catalytic material (mesoporous TiO2 films) for the cathodic detection of hydrogen peroxide in air-saturated aqueous solution is described. The immobilization of glucose oxidase (GOD) as enzyme model onto the TiO2 electrode provides an efficient biosensor for the amperometric detection of glucose at −0.15 V (vs. SCE). GOD molecules were immobilized in an inorganic laponite gel and cross-linked by glutaraldehyde. The highest sensitivity and detection limit were 3.33 mAM−1 cm−2 and 15 μM, respectively. In addition different procedures of enzyme immobilization including cross-linking with glutaraldehyde and physical entrapment in a functionalized polypyrrole film have been compared.