Full Paper

A Disposable Biosensor for Organophosphorus Nerve Agents Based on Carbon Nanotubes Modified Thick Film Strip Electrode

  1. Kanchan A. Joshi1,
  2. Jason Tang1,
  3. Robert Haddon1,2,
  4. Joseph Wang3,
  5. Wilfred Chen1,
  6. Ashok Mulchandani1

Article first published online: 20 OCT 2004

DOI: 10.1002/elan.200403118

Issue

Electroanalysis

Electroanalysis

Volume 17, Issue 1, pages 54–58, January 2005

Additional Information

How to Cite

Joshi, Kanchan A., Tang, J., Haddon, R., Wang, J., Chen, W. and Mulchandani, A. (2005), A Disposable Biosensor for Organophosphorus Nerve Agents Based on Carbon Nanotubes Modified Thick Film Strip Electrode. Electroanalysis, 17: 54–58. doi: 10.1002/elan.200403118

Author Information

  1. 1

    Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA

  2. 2

    Department of Chemistry, University of California, Riverside, CA 92521, USA

  3. 3

    Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003, USA

Publication History

  1. Issue published online: 18 JAN 2005
  2. Article first published online: 20 OCT 2004
  3. Manuscript Accepted: 23 JUN 2004
  4. Manuscript Received: 4 MAY 2004

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (77K)

Keywords:

  • Carbon nanotubes;
  • Thick film strip electrodes;
  • Biosensor;
  • Organophosphorus insecticides;
  • Acetylcholinesterase;
  • Paraoxons

Abstract

A disposable biosensor based on acetylcholinesterase-functionalized acid purified multi-wall carbon nanotubes (CNTs) modified thick film strip electrode for organophosphorus (OP) insecticides was developed. The degree of inhibition of the enzyme acteylcholinesterase (AChE) by OP compounds was determined by measuring the electrooxidation current of the thiocholine generated by the AChE catalyzed hydrolysis of acteylthiocholine (ATCh). The large surface area and electro-catalytic activity of carbon nanotubes lowered the overpotential for thiocholine oxidation to 200 mV (vs. Ag/AgCl) without the use of mediating redox species and enzyme immobilization by physical adsorption. The biosensor detected as low as 0.5 nM (0.145 ppb) of the model organophosphate nerve agent paraoxon with good precision, electrode to electrode reproducibility and stability. Analysis of real water sample using the sensor demonstrated the feasibility of the application of the sensor for on site monitoring of OP compounds.

Get PDF (77K)