Full Paper

High Sensitive Electrochemical Detection of Sequence-Specific DNA Using Low Current Voltammetry

  1. Lingling Lin,
  2. Jianzhang Zhou,
  3. Yan Zhang,
  4. Zhonghua Lin

Article first published online: 25 JUN 2008

DOI: 10.1002/elan.200804248

Issue

Electroanalysis

Electroanalysis

Volume 20, Issue 16, pages 1798–1804, August 2008

Additional Information

How to Cite

Lin, L., Zhou, J., Zhang, Y. and Lin, Z. (2008), High Sensitive Electrochemical Detection of Sequence-Specific DNA Using Low Current Voltammetry. Electroanalysis, 20: 1798–1804. doi: 10.1002/elan.200804248

Author Information

  1. State Key Laboratory of Physical Chemistry of the Solid Surface and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, P. R.China

Publication History

  1. Issue published online: 13 AUG 2008
  2. Article first published online: 25 JUN 2008
  3. Manuscript Accepted: 30 APR 2008
  4. Manuscript Received: 10 MAR 2008

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Keywords:

  • Electrochemical detection;
  • Sandwich-type DNA;
  • Mismatch;
  • False positive hybridization;
  • Deformability

Abstract

In this article, we report on efforts to construct a high sensitive electrochemical sensor with immobilized sandwich-type DNA borne ferrocene (Fc) head for sequence-specific DNA detection using ultramicroelectrode and low current voltammetry. Based on the difference in deformability between the bending rigid complementary DNA double helix and its anomalous flexile mismatches, the fully complementary target can be distinguished from mismatched targets including the single-base mismatched target. Detection limit estimated as the amount of DNA is observed to be 100 fM via low current voltammetry. The method offers great promise of high sensitivity and selectivity simultaneously for effective gene identification.

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