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Molecularly Imprinted Polymerized Methylene Green as a Platform for Electrochemical Sensing of Aptamer–Thrombin Interactions

  1. Gennady Evtugyn1,
  2. Anna Porfireva1,
  3. Alexey Ivanov1,3,
  4. Olga Konovalova2,
  5. Tibor Hianik3

Article first published online: 29 APR 2009

DOI: 10.1002/elan.200804556

Issue

Electroanalysis

Electroanalysis

Special Issue: Aptamer-Based Electrochemical Biosensors

Volume 21, Issue 11, pages 1272–1277, June 2009

Additional Information

How to Cite

Evtugyn, G., Porfireva, A., Ivanov, A., Konovalova, O. and Hianik, T. (2009), Molecularly Imprinted Polymerized Methylene Green as a Platform for Electrochemical Sensing of Aptamer–Thrombin Interactions. Electroanalysis, 21: 1272–1277. doi: 10.1002/elan.200804556

Author Information

  1. 1

    Analytical Chemistry Department

  2. 2

    Department of Optics and Nanophotonics, Kazan State University, 18 Kremlevskaya Street, Kazan, 420008, Russian Federation

  3. 3

    Department of Nuclear Physics and Biophysics, Comenius University, Mlynska dolina F1, 842 48 Bratislava, Slovakia

Publication History

  1. Issue published online: 28 MAY 2009
  2. Article first published online: 29 APR 2009
  3. Manuscript Accepted: 8 DEC 2008
  4. Manuscript Received: 6 NOV 2008

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Keywords:

  • DNA;
  • Aptamers;
  • Thrombin;
  • Molecularly imprinted polymers;
  • Methylene green;
  • Electrochemical impedance spectroscopy

Abstract

The biosensors based on DNA aptamers immobilized by electrostatic adsorption onto electropolymerized Methylene Green (MG)) imprinted with DNA have been developed and examined for thrombin detection in the range from 1 nM to 1 μM using electrochemical impedance spectroscopy (EIS) and potentiometry. The participation of DNA in MG electropolymerization and imprints formation was proved by measurements of EIS and redox signals of neutral and charged indicators. Changes in the morphology of the layers were examined using atomic force microscopy (AFM). The addition of DNA at the electropolymerization stage followed by acidic treatment of the coating significantly improved the efficiency of electrostatic adsorption of the DNA aptamer and provided sensitive detection of thrombin with limit of detection 0.5 nM.

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