Section A: Enzyme-Based Biofuel Cells

Enzymatic Anodes for Hydrogen Fuel Cells based on Covalent Attachment of Ni-Fe Hydrogenases and Direct Electron Transfer to SAM-Modified Gold Electrodes

  1. Olaf Rüdiger1,4,
  2. Cristina Gutiérrez-Sánchez1,
  3. David Olea1,
  4. Inês A. C. Pereira2,
  5. Marisela Vélez1,3,
  6. Víctor M. Fernández1,
  7. Antonio L. De Lacey1

Article first published online: 9 MAR 2010

DOI: 10.1002/elan.200880002

Issue

Electroanalysis

Electroanalysis

Special Issue: Biofuel Cells

Volume 22, Issue 7-8, pages 776–783, April 2010

Additional Information

How to Cite

Rüdiger, O., Gutiérrez-Sánchez, C., Olea, D., Pereira, Inês A. C., Vélez, M., Fernández, Víctor M. and De Lacey, Antonio L. (2010), Enzymatic Anodes for Hydrogen Fuel Cells based on Covalent Attachment of Ni-Fe Hydrogenases and Direct Electron Transfer to SAM-Modified Gold Electrodes. Electroanalysis, 22: 776–783. doi: 10.1002/elan.200880002

Author Information

  1. 1

    Instituto de Catálisis y Petroleoquímica, CSIC, c/ Marie Curie 2, 28049 Madrid, Spain

  2. 2

    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal

  3. 3

    Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA-Nanociencia), Facultad de Ciencias, C-IX-3a Cantoblanco, 28049 Madrid, Spain

  4. 4

    Max-Planck-Institut für Bioanorganische Chemie, Stiftstrasse 34-36, D-45470 Müllheim an der Ruhr, Germany

Publication History

  1. Issue published online: 1 APR 2010
  2. Article first published online: 9 MAR 2010
  3. Manuscript Accepted: 1 OCT 2009
  4. Manuscript Received: 15 JUN 2008

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Keywords:

  • Hydrogenases;
  • Biofuel cells;
  • SAM;
  • Direct electron transfer;
  • AFM;
  • Fuel cells;
  • Enzymes

Abstract

Immobilization of hydrogenases onto electrodes is of great interest for developing biofuel cells that use H2 as a fuel. In this way, hydrogenases replace Pt as electrocatalyst for oxidizing H2 in the anode. We have developed a method of covalent bonding of Desulfovibrio gigas Ni-Fe hydrogenase and Desulfovibrio vulgaris Hildenborough Ni-Fe-Se hydrogenase to gold electrodes modified with a self assembled monolayer (SAM) of 4-aminothiophenol for measuring high electrocatalytic currents of H2-oxidation in the absence of redox mediators. Electrochemical measurements and atomic force microscopy characterization show that direct electron transfer between enzyme and the Au support is due to formation of an organized monolayer of hydrogenase over the SAM-modified surface.

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