Section B: Microbial Biofuel Cells

Single Cell Isolation of Bacteria from Microbial Fuel Cells and Potomac River Sediment

  1. B. R. Ringeisen1,
  2. S. E. Lizewski1,
  3. L. A. Fitzgerald1,
  4. J. C. Biffinger1,
  5. C. L. Knight2,
  6. W. J. Crookes-Goodson2,
  7. P. K. Wu3

Article first published online: 9 MAR 2010

DOI: 10.1002/elan.200880012

Issue

Electroanalysis

Electroanalysis

Special Issue: Biofuel Cells

Volume 22, Issue 7-8, pages 875–882, April 2010

Additional Information

How to Cite

Ringeisen, B. R., Lizewski, S. E., Fitzgerald, L. A., Biffinger, J. C., Knight, C. L., Crookes-Goodson, W. J. and Wu, P. K. (2010), Single Cell Isolation of Bacteria from Microbial Fuel Cells and Potomac River Sediment. Electroanalysis, 22: 875–882. doi: 10.1002/elan.200880012

Author Information

  1. 1

    Chemistry Division, Naval Research Laboratory, Washington, DC 20375, USA

  2. 2

    Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH 45433, USA

  3. 3

    Physics Department, Southern Oregon University, Ashland, OR 97520, USA

Publication History

  1. Issue published online: 1 APR 2010
  2. Article first published online: 9 MAR 2010
  3. Manuscript Accepted: 8 OCT 2009
  4. Manuscript Received: 15 JUN 2008

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Keywords:

  • Biological laser printing (BioLP);
  • Cell sorting;
  • Cell printing;
  • Electrochemically active bacteria (EAB);
  • Microbial fuel cell (MFC);
  • Potomac River sediment;
  • Shewanella oneidensis;
  • Fuel cells

Abstract

Electrochemically active bacteria (EAB) are prominently found in aquatic environmental sediment samples and wastewater streams, which are known to contain several different types of microorganisms. Even though microbial consortia are found to enhance both Coulombic efficiency and total power output in microbial fuel cells (MFCs), it is currently unknown how many different EAB contribute to current generation in these systems. It is also difficult to track the relative population of different species during MFC operation. We used biological laser printing (BioLP) to isolate different bacterium from complex environmental samples and MFC anolytes. BioLP can be used to print droplets containing a single cell directly from liquid culture, thereby enabling EAB to be sorted from unmodified environmental or MFC samples. Isolated species were identified through 16S rDNA analysis of pure cultures derived from the printed samples. These experiments demonstrate how cell printing can be used as a single-step method to separate and identify microorganisms from complex environmental samples and operating MFCs.

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