Short Communication

Streptavidin Modified Carbon Nanotube Based Graphite Electrode for Label-Free Sequence Specific DNA Detection

  1. Arzum Erdem1,
  2. Pagona Papakonstantinou2,
  3. Hayley Murphy2,
  4. Martin McMullan2,
  5. Hakan Karadeniz1,
  6. Surbhi Sharma2

Article first published online: 3 MAR 2010

DOI: 10.1002/elan.200900436

Issue

Electroanalysis

Electroanalysis

Volume 22, Issue 6, pages 611–617, March 2010

Additional Information

How to Cite

Erdem, A., Papakonstantinou, P., Murphy, H., McMullan, M., Karadeniz, H. and Sharma, S. (2010), Streptavidin Modified Carbon Nanotube Based Graphite Electrode for Label-Free Sequence Specific DNA Detection. Electroanalysis, 22: 611–617. doi: 10.1002/elan.200900436

Author Information

  1. 1

    Ege University, Faculty of Pharmacy, Analytical Chemistry Department, 35100 Bornova, Izmir, Turkey

  2. 2

    Nanotechnology and Integrated BioEngineering Centre, NIBEC, School of Electrical and Mechanical Engineering, Newtownabbey, Co. Antrim BT37OQB, N. Ireland, UK

Publication History

  1. Issue published online: 3 MAR 2010
  2. Article first published online: 3 MAR 2010
  3. Manuscript Accepted: 6 DEC 2009
  4. Manuscript Received: 1 SEP 2009

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Keywords:

  • Multiwalled carbon nanotube;
  • Biotin-streptavidin;
  • DNA hybridization;
  • guanine signal;
  • Electrochemical impedance spectroscopy;
  • Nanotubes

Abstract

Disposable graphite pencil electrodes (PGE) modified with multiwalled carbon nanotubes (MWCNTs)-streptavidin (STR) conjugates were used for electrochemical monitoring of label-free DNA hybridization. The surface morphology of PGE electrode before and after hybridization was characterized by scanning electron microscopy. Electrochemical impedance spectroscopy was used to monitor each step of the construction of the DNA biosensor. The biosensor was demonstrated to have excellent selectivity, being able to differentiate complementary sequences from a noncomplementary ones and in addition select the target sequence of DNA from a mixture of other DNA without loss in current sensitivity.

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