A Uniquely Configured Acetylcholinesterase-Lecithin Biomimetic Sensing Structure at Cyclohexane/Water Interface for Electrochemical Determination of Fenthion in Organic Solvent

Authors

  • Jun Li,

    1. Lab of Biomimetic Electrochemistry and Biosensors, College of Chemistry and Life Sciences, Institute of Physical Chemistry, Zhejiang Normal University, Jinhua 321004, P. R. China
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  • Jingjing He,

    1. Lab of Biomimetic Electrochemistry and Biosensors, College of Chemistry and Life Sciences, Institute of Physical Chemistry, Zhejiang Normal University, Jinhua 321004, P. R. China
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  • Jingxia Qiu,

    1. Lab of Biomimetic Electrochemistry and Biosensors, College of Chemistry and Life Sciences, Institute of Physical Chemistry, Zhejiang Normal University, Jinhua 321004, P. R. China
    2. Griffith School of Environment and Environmental Futures Center, Gold Coast Campus, Griffith University, QLD 4222, Australia
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  • Shanqing Zhang,

    1. Griffith School of Environment and Environmental Futures Center, Gold Coast Campus, Griffith University, QLD 4222, Australia
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  • Huijun Zhao,

    1. Griffith School of Environment and Environmental Futures Center, Gold Coast Campus, Griffith University, QLD 4222, Australia
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  • Yuqing Miao

    1. Lab of Biomimetic Electrochemistry and Biosensors, College of Chemistry and Life Sciences, Institute of Physical Chemistry, Zhejiang Normal University, Jinhua 321004, P. R. China
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Abstract

An acetylcholinesterase (AChE)-lecithin biomimetic structure was constructed at the oil/water interface for the direct determination of fenthion in cyclohexane. Indophenol acetate in oil phase was hydrolyzed by AChE at the two-phase interface to produce indophenol. Square wave voltammetry was used to monitor the current decrease of substrate to assess AChE activity. The AChE incorporated in the lecithin-based biomimetic layer possessed higher enzymatic activity and was more sensitive to fenthion inhibition than freestanding AChE. A linear relationship between the inhibition percentage and logarithm of fenthion concentration was obtained in a concentration range from 1 ng/mL to 1 mg/mL.

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