Development of a Glucose Biosensor Using Advanced Electrode Modified by Nanohybrid Composing Chemically Modified Graphene and Ionic Liquid

Authors

  • Min Ho Yang,

    1. Department of Chemical & Biomolecular Engineering (BK21 program), KAIST, Daejeon 305-701, Korea
    2. BioProcess Engineering Research Center, Center for Systems & Synthetic Biotechnology, and Institute for the BioCentury, KAIST, Daejeon 305-701, Korea
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    • Min Ho Yang and Bong Gil Choi contributed equally to this work.

  • Bong Gill Choi,

    1. Department of Chemical & Biomolecular Engineering (BK21 program), KAIST, Daejeon 305-701, Korea
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    • Min Ho Yang and Bong Gil Choi contributed equally to this work.

  • HoSeok Park,

    1. Department of Chemical Engineering, Kyung Hee University, Yongin-si 446-701, Korea
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  • Won Hi Hong,

    1. Department of Chemical & Biomolecular Engineering (BK21 program), KAIST, Daejeon 305-701, Korea
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  • Sang Yup Lee,

    1. Department of Chemical & Biomolecular Engineering (BK21 program), KAIST, Daejeon 305-701, Korea
    2. BioProcess Engineering Research Center, Center for Systems & Synthetic Biotechnology, and Institute for the BioCentury, KAIST, Daejeon 305-701, Korea
    3. Department of Bio & Brain Engineering, Department of Biological Sciences, and Bioinformatics Research Center, KAIST, Daejeon 305-701, Korea
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  • Tae Jung Park

    1. BioProcess Engineering Research Center, Center for Systems & Synthetic Biotechnology, and Institute for the BioCentury, KAIST, Daejeon 305-701, Korea
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Abstract

Nanohybrids of chemically modified graphene (CMG) and ionic liquid (IL) were prepared by sonication to modify the electrode. The modified CMG-IL electrodes showed a higher current and smaller peak-to-peak potential separation than a bare electrode due to the promoted electron transfer rate. Furthermore, the glucose oxidase (GOx) immobilized on the modified electrode displayed direct electron transfer rate and symmetrical redox potentials with a linear relationship at different scan rates. The fabricated GOx/CMG-IL electrodes were developed selective glucose biosensor with respect to a sensitivity of 0.64 μA mM−1, detection limit of 0.376 mM, and response time of <5 s.

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