Determination of Ofloxacin in Pharmaceuticals, Human Urine and Serum Using a Potentiometric Sensor

Authors

  • A. M. Pimenta,

    Corresponding author
    1. REQUIMTE, CEBIMED, Faculdade de Ciências da Saúde, Universidade Fernando Pessoa, Rua Carlos da Maia, 296, 4200-150 Porto, Portugal
    • REQUIMTE, CEBIMED, Faculdade de Ciências da Saúde, Universidade Fernando Pessoa, Rua Carlos da Maia, 296, 4200-150 Porto, Portugal
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  • M. R. S. Souto,

    1. CBQF, CEBIMED, Faculdade de Ciências da Saúde, Universidade Fernando Pessoa, Rua Carlos da Maia, 296, 4200-150 Porto, Portugal
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  • R. I. L. Catarino,

    1. REQUIMTE, CEBIMED, Faculdade de Ciências da Saúde, Universidade Fernando Pessoa, Rua Carlos da Maia, 296, 4200-150 Porto, Portugal
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  • M. F. C. Leal,

    1. REQUIMTE, CEBIMED, Faculdade de Ciências da Saúde, Universidade Fernando Pessoa, Rua Carlos da Maia, 296, 4200-150 Porto, Portugal
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  • J. L. F. C. Lima

    1. REQUIMTE, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha 164, 4090-030 Porto, Portugal
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Abstract

An ofloxacin-selective electrode was developed for application in pharmaceutical preparations, serum and urine analysis. The electrode is based on tetrakis[3,5-bis(trifluoromethyl)phenyl]borate as ion exchanger in a poly(vinyl chloride) membrane. The sensor displayed a Nernstian response for ofloxacin over a wide concentration range (3×10−6–1×10−2 mol L−1) with a slope of 55±1 mV per decade of concentration and a practical detection limit of 1×10−6 mol L−1. The electrode was successfully applied to the determination of ofloxacin in samples with no pretreatment steps, adequate accuracy and precision and recoveries within the intervals of 94–107 %.

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