Direct Electrochemistry and Electrocatalysis of Horseradish Peroxidase Immobilized on Water Soluble Sulfonated Graphene Film via Self-assembly

Authors

  • Qian Zhang,

    1. Liaoning Provincial Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials, Liaoning University, Shenyang 110036, P. R. China
    2. College of Chemistry, Liaoning University, Shenyang 110036, P. R. China
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  • Yun Qiao,

    1. Liaoning Provincial Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials, Liaoning University, Shenyang 110036, P. R. China
    2. College of Chemistry, Liaoning University, Shenyang 110036, P. R. China
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  • Ling Zhang,

    1. College of Chemistry and Life Science, Shenyang Normal University, Shenyang 110034, P. R. China
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  • Shuyao Wu,

    1. Liaoning Provincial Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials, Liaoning University, Shenyang 110036, P. R. China
    2. College of Chemistry, Liaoning University, Shenyang 110036, P. R. China
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  • Hua Zhou,

    Corresponding author
    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China
    • State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China
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  • Jingwei Xu,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China
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  • Xi-Ming Song

    Corresponding author
    1. Liaoning Provincial Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials, Liaoning University, Shenyang 110036, P. R. China
    2. College of Chemistry, Liaoning University, Shenyang 110036, P. R. China
    • Liaoning Provincial Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials, Liaoning University, Shenyang 110036, P. R. China
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Abstract

A novel water soluble sulfonated graphene (denoted as sulfonated-G), was synthesized for the immobilization and biosensing of horseradish peroxidase (HRP). Compared with unmodified graphene, sulfonated-G could not only well disperse in aqueous solution as individual nanosheet, but also exhibit apparent negative charge. Using a simple self-assembly method, HRP was immobilized on sulfonated-G at the surface of GC electrode to form HRP/sulfonated-G/GC electrode under mild condition. The immobilized HRP showed enhanced direct electron transfer for the HRP-Fe(III)/Fe(II) redox centre. Based on the direct electron transfer of the immobilized HRP, HRP/sulfonated-G/GC electrode exhibited excellent catalytic performance for H2O2 and NaNO2 reduction. Accordingly, the proposed method not only simplifies the methodology of enzyme immobilization, but also provides an efficient platform for fabricating novel biosensors and bioelectronic devices.

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