Methomyl Detection by Inhibition of Laccase Using a Carbon Ceramic Biosensor

Authors

  • Suellen Cadorin Fernandes,

    Corresponding author
    1. Departamento de Química, Universidade Federal de Santa Catarina, 88040-970, Florianópolis, SC, Brazil tel.:+55 48 3721 6844; fax:+55 48 3721 6850
    • Departamento de Química, Universidade Federal de Santa Catarina, 88040-970, Florianópolis, SC, Brazil tel.:+55 48 3721 6844; fax:+55 48 3721 6850
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  • Iolanda Cruz Vieira,

    1. Departamento de Química, Universidade Federal de Santa Catarina, 88040-970, Florianópolis, SC, Brazil tel.:+55 48 3721 6844; fax:+55 48 3721 6850
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  • Antonio Marcos Jacques Barbosa,

    1. Departamento de Química, Universidade Federal do Mato Grosso do Sul, 79070-900 – Campo Grande, MS, Brazil
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  • Valdir Souza Ferreira

    1. Departamento de Química, Universidade Federal do Mato Grosso do Sul, 79070-900 – Campo Grande, MS, Brazil
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Abstract

A sol–gel derived carbon ceramic biosensor was used for methomyl determination in vegetable extract samples based on the immobilization of laccase from Aspergillus oryzae. Esculetin was chosen as the substrate for laccase in order to measure inhibition by this pesticide. The analytical curve was linear for methomyl concentrations of 0.5 to 12.2 µM with a detection limit of 0.2 µM. The lifetime of the proposed biosensor was 60 days and the recovery from vegetable extract samples ranged from 98.0 to 104.2 %. The results using the proposed method are in agreement with those using HPLC at the 95 % confidence level.

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