Multioperational Oxidase Biosensors Based on Carbosilane Dendrimers with Interacting Ferrocenes

Authors

  • M. García Martínez,

    1. Departamento de Ingeniería Química Industrial, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, José Gutiérrez Abascal 2, 28006-Madrid, Spain phone: +34 91 336 31 85; fax: +34 91 336 30 09
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  • B. Alonso,

    1. Departamento de Química Inorgánica, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco 28049-Madrid, Spain
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  • C. M. Casado,

    1. Departamento de Química Inorgánica, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco 28049-Madrid, Spain
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  • J. Losada,

    Corresponding author
    1. Departamento de Ingeniería Química Industrial, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, José Gutiérrez Abascal 2, 28006-Madrid, Spain phone: +34 91 336 31 85; fax: +34 91 336 30 09
    • Departamento de Ingeniería Química Industrial, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, José Gutiérrez Abascal 2, 28006-Madrid, Spain phone: +34 91 336 31 85; fax: +34 91 336 30 09
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  • M. P. García Armada

    Corresponding author
    1. Departamento de Ingeniería Química Industrial, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, José Gutiérrez Abascal 2, 28006-Madrid, Spain phone: +34 91 336 31 85; fax: +34 91 336 30 09
    • Departamento de Ingeniería Química Industrial, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, José Gutiérrez Abascal 2, 28006-Madrid, Spain phone: +34 91 336 31 85; fax: +34 91 336 30 09
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Abstract

The bioelectrocatalytical properties and kinetic characteristics of new oxidase biosensors based on two different carbosilane dendrimers are described. The best glucose biosensor developed displayed, in an ascorbate interference free work potential interval, a strictly linear range from 0 to 4.0 mM, a detection limit of 40,6 μM and a response time less than 3 s. The lactate biosensor displayed a linear range from 0 to 0.8 mM, a detection limit of 0.73 µM and a response time less than 2 s. The apparent Michaelis–Menten constants were calculated to be 4.39 mM and 2.08 mM respectively, according to Lineweaver–Burk equation.

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