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Gold-Decorated Carbon Composite Electrodes for Enzymatic Oxygen Reduction

Authors

  • Heather R. Luckarift,

    Corresponding author
    1. Air Force Research Laboratory, AFRL/RXQL, Tyndall Air Force Base, FL, USA 32403
    2. Universal Technology Corporation, 1270 N. Fairfield Rd, Dayton, OH, USA 45432
    • Air Force Research Laboratory, AFRL/RXQL, Tyndall Air Force Base, FL, USA 32403
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  • Dmitri M. Ivnitski,

    1. Center for Emerging Energy Technologies and Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, NM, USA 87131
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    • Khripin now at NIST polymer division, Gaithersburg, MA, USA 20899; Ivnitski now at Department of Chemical Engineering, Columbia University, New York, NY, USA 10027

  • Carolin Lau,

    1. Center for Emerging Energy Technologies and Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, NM, USA 87131
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  • Constantine Khripin,

    1. Center for Emerging Energy Technologies and Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, NM, USA 87131
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    • Khripin now at NIST polymer division, Gaithersburg, MA, USA 20899; Ivnitski now at Department of Chemical Engineering, Columbia University, New York, NY, USA 10027

  • Plamen Atanassov,

    1. Center for Emerging Energy Technologies and Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, NM, USA 87131
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  • Glenn R. Johnson

    Corresponding author
    1. Air Force Research Laboratory, AFRL/RXQL, Tyndall Air Force Base, FL, USA 32403
    • Air Force Research Laboratory, AFRL/RXQL, Tyndall Air Force Base, FL, USA 32403
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Abstract

Functional composites of carbon and gold nanoparticles create a hierarchical architecture that facilitates high enzyme loading. Subsequent immobilization of the multicopper oxidase, Trametes versciolor laccase, was optimal with dithiobis- (succinimidyl propionate), due to the formation of thiol bonds between the protein molecules and gold. The immobilized laccase catalyzed oxygen reduction, with an onset potential of ∼0.6 V (vs. Ag/AgCl), indicated effective orientation of the enzyme redox center to enable direct electron transport between enzyme and the composite electrode. Current densities in half-cell configurations provide scalable outputs of 50–80 µA/cm2 with the optimized electrode design. The methodology herein describes a rapid, facile preparation of gold-decorated carbon composite materials for use as electrode scaffolds that can be integrated into a range of bioelectronic devices.

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