Get access

Study of Inhibition, Reactivation and Aging Processes of Pesticides Using Graphene Nanosheets/Gold Nanoparticles-Based Acetylcholinesterase Biosensor

Authors

  • Lin Zhang,

    1. Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079, P. R. China tel/fax: +86-27-67867958
    Search for more papers by this author
  • Linjuan Long,

    1. Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079, P. R. China tel/fax: +86-27-67867958
    Search for more papers by this author
  • Weiying Zhang,

    1. Pacific Northwest National Laboratory, Richland, Washington, 99352, USA
    Search for more papers by this author
  • Dan Du,

    Corresponding author
    1. Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079, P. R. China tel/fax: +86-27-67867958
    • Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079, P. R. China tel/fax: +86-27-67867958
    Search for more papers by this author
  • Yuehe Lin

    Corresponding author
    1. Pacific Northwest National Laboratory, Richland, Washington, 99352, USA
    • Pacific Northwest National Laboratory, Richland, Washington, 99352, USA
    Search for more papers by this author

Abstract

Organophosphate (OP) and carbamate pesticides exert their toxicity via attacking the hydroxyl moiety of serine in the ‘active site’ of acetylcholinesterase (AChE). In this paper we developed a stable AChE biosensor based on self-assembling AChE to graphene nanosheet (GN)-gold nanoparticles (AuNPs) nanocomposite electrode for investigation of inhibition, reactivation and aging processes of different pesticides. It is confirmed that pesticides can inhibit AChE in a short time. OPs poisoning is treatable with oximes while carbarmates exposure is insensitive to oximes. The proposed electrochemical approach thus provides a new simple tool for comparison of pesticide sensitivity and guide of therapeutic intervention.

Get access to the full text of this article

Ancillary