Development of Amperometric Glucose Biosensor Based on Reconstitution of Glucose Oxidase on Polymeric 3-Aminophenyl Boronic Acid Monolayer

Authors

  • Mehmet Şenel,

    Corresponding author
    1. Department of Chemistry, Faculty of Arts and Sciences, Fatih University, Buyukcekmece Kampusu, 34500 B.Cekmece, Istanbul 34500, Turkey, Tel: +902128663300; Fax: +902128663402
    • Department of Chemistry, Faculty of Arts and Sciences, Fatih University, Buyukcekmece Kampusu, 34500 B.Cekmece, Istanbul 34500, Turkey, Tel: +902128663300; Fax: +902128663402
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  • Cevdet Nergiz,

    Corresponding author
    1. Department of Chemistry, Faculty of Arts and Sciences, Fatih University, Buyukcekmece Kampusu, 34500 B.Cekmece, Istanbul 34500, Turkey, Tel: +902128663300; Fax: +902128663402
    • Department of Chemistry, Faculty of Arts and Sciences, Fatih University, Buyukcekmece Kampusu, 34500 B.Cekmece, Istanbul 34500, Turkey, Tel: +902128663300; Fax: +902128663402
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  • Muamer Dervisevic,

    1. Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500,Turkey
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  • Emre Çevik

    1. Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500,Turkey
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Abstract

An amperometric biosensor for determining glucose based on deflavination of the enzyme glucose oxidase and subsequent reconstitution of the apo-protein with a complexed flavin adenine dinucleotide (FAD) monolayer is described. The GOx-reconstituted electrode exhibited excellent electrocatalytic activities towards the reduction and oxidation of hydrogen peroxide as well. The prepared biosensor showed an excellent performance for glucose at +0.5 V with a high sensitivity (5.94 μA/mM) and relatively good response time (∼12 s) in a wide concentration range of 1–17 mM (correlation coefficient of 0.9998). The applicability to blood analysis was also evaluated.

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