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Keywords:

  • Polyaniline;
  • Glucose Oxidase;
  • Biosensor;
  • Boronic acid

Abstract

An amperometric biosensor for determining glucose based on deflavination of the enzyme glucose oxidase and subsequent reconstitution of the apo-protein with a complexed flavin adenine dinucleotide (FAD) monolayer is described. The GOx-reconstituted electrode exhibited excellent electrocatalytic activities towards the reduction and oxidation of hydrogen peroxide as well. The prepared biosensor showed an excellent performance for glucose at +0.5 V with a high sensitivity (5.94 μA/mM) and relatively good response time (∼12 s) in a wide concentration range of 1–17 mM (correlation coefficient of 0.9998). The applicability to blood analysis was also evaluated.