The Enhanced Enzymolysis Resistance of Surface-Immobilized DNA Caused by Hybridizing with Morpholino

Authors

  • Lei Cao,

    1. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China tel & fax: +86-25-83685947
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  • Yun Zhao,

    1. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China tel & fax: +86-25-83685947
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  • Li-Na Ji,

    1. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, P. R. China
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  • Jing Zhang,

    1. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China tel & fax: +86-25-83685947
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  • Kang Wang,

    Corresponding author
    1. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China tel & fax: +86-25-83685947
    • State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China tel & fax: +86-25-83685947
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  • Xing-Hua Xia

    1. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China tel & fax: +86-25-83685947
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Abstract

The anti-enzymolysis capability of surface attached DNA, morpholino (MO) single strands, and DNA-DNA, MO-DNA double strands are studied using cyclic voltammetry. Results show that MO, the neutral analog of DNA, bears strong anti-enzymolysis capability against DNase I and can protect its complementary DNA from being decomposed. Only 15 % of the DNA in the MO-DNA double strands is decomposed within 20 min, which is much lower than that for DNA-DNA double strands (100 % in 400 s). Fluorescence polarization measurements suggest that the steric hindrance of MO is the main cause of the improved anti-enzymolysis ability of DNA in MO-DNA double strands.

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