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Direct Electrochemistry of Glucose Oxidase at Reduced Graphene Oxide and β-Cyclodextrin Composite Modified Electrode and Application for Glucose Biosensing

Authors

  • Selvakumar Palanisamy,

    1. Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan (R.O.C) tel: +886 2270 17147; fax: +886 2270 25238
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  • Rajkumar Devasenathipathy,

    1. Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan (R.O.C) tel: +886 2270 17147; fax: +886 2270 25238
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  • Shen-Ming Chen,

    Corresponding author
    1. Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan (R.O.C) tel: +886 2270 17147; fax: +886 2270 25238
    2. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia

    • Shen-Ming Chen, Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan (R.O.C) tel: +886 2270 17147; fax: +886 2270 25238

      P. Prakash, Department of Chemistry, Thiagarajar College, Madurai-625009, Tamilnadu, India tel: +919842993931; fax: +914522312375

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  • M. Ajmal Ali,

    1. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
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  • Chelladurai Karuppiah,

    1. Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan (R.O.C) tel: +886 2270 17147; fax: +886 2270 25238
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  • V. Balakumar,

    1. Department of Chemistry, Thiagarajar College, Madurai-625009, Tamilnadu, India tel: +919842993931; fax: +914522312375
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  • P. Prakash,

    Corresponding author
    1. Department of Chemistry, Thiagarajar College, Madurai-625009, Tamilnadu, India tel: +919842993931; fax: +914522312375

    • Shen-Ming Chen, Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan (R.O.C) tel: +886 2270 17147; fax: +886 2270 25238

      P. Prakash, Department of Chemistry, Thiagarajar College, Madurai-625009, Tamilnadu, India tel: +919842993931; fax: +914522312375

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  • Mohamed S. Elshikh,

    1. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
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  • Fahad M. A. Al-Hemaid

    1. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
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Abstract

A simple glucose biosensor has been developed based on direct electrochemistry of glucose oxidase (GOx) immobilized on the reduced graphene oxide (RGO) and β-cyclodextrin (CD) composite. A well-defined redox couple of GOx appears with a formal potential of ∼−0.459 V at RGO/CD composite. A heterogeneous electron transfer rate constant (Ks) has been calculated for GOx at RGO/CD as 3.8 s−1. The fabricated biosensor displays a wide response to glucose in the linear concentrations range from 50 µM to 3.0 mM. The sensitivity and limit of detection of the biosensor is estimated as 59.74 µA mM−1 cm−2 and 12 µM, respectively.

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DOI

10.1002/elan.201500150

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© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

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Keywords

  • Reduced graphene oxide;
  • β-cyclodextrin;
  • Glucose oxidase;
  • Direct electrochemistry;
  • Glucose biosensor

Publication History

  • Issue online:
  • Version of record online:
  • Manuscript Accepted:
  • Manuscript Received:

Funded by

  • Visiting Professorship to SMC at King Saud University

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  1. 1John H.T. Luong, Jeremy D. Glennon, Aharon Gedanken, Sandeep K. Vashist, Achievement and assessment of direct electron transfer of glucose oxidase in electrochemical biosensing using carbon nanotubes, graphene, and their nanocomposites, Microchimica Acta, 2017, 184, 2, 369CrossRef
  2. 2Wajee Jaikaew, Rungrueang Patanakul, Albert Schulte, Electrical Cable-based Copper Disk Electrodes as Oxidase Biosensor Platforms with Cathodic H2 O2 Readout, Electroanalysis, 2016, 28, 10, 2408Wiley Online Library
  3. 3Kokulnathan Thangavelu, Selvakumar Palanisamy, Shen-Ming Chen, Vijayalakshmi Velusamy, Tse-Wei Chen, Sayee Kannan Ramaraj, Electrochemical Determination of Caffeic Acid in Wine Samples Using Reduced Graphene Oxide/Polydopamine Composite, Journal of The Electrochemical Society, 2016, 163, 14, B726CrossRef
  4. 4Kathiresan Vijayaraj, Suck Won Hong, Sung-Ho Jin, Seung-Cheol Chang, Deog-Su Park, Fabrication of a novel disposable glucose biosensor using an electrochemically reduced graphene oxide–glucose oxidase biocomposite, Anal. Methods, 2016, 8, 38, 6974CrossRef