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Article
Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins
Article first published online: 13 APR 2005
DOI: 10.1002/elps.1150070108
Copyright © 1986 VCH Verlagsgesellschaft mbH
1522-2683/asset/cover.gif?v=1&s=a46778197acda8c447d4e2dcfc227b0d99b34430 "ELECTROPHORESIS")
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How to Cite
Damerval, C., De Vienne, D., Zivy, M. and Thiellement, H. (1986), Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins. ELECTROPHORESIS, 7: 52–54. doi: 10.1002/elps.1150070108
Publication History
- Issue published online: 13 APR 2005
- Article first published online: 13 APR 2005
- Manuscript Received: 23 SEP 1985
- Abstract
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- Cited By
Abstract
The level of genetic variation revealed by two-dimensional electrophoresis of proteins from seedlings of two wheat lines strongly depends on the technical procedures. Improvements in extraction and electrophoresis procedures relative to earlier experiments on the same material led to a significant increase in the genetic variation revealed: 15.2 % instead of 6.7 % of the spots were genetically variable. The improved procedure is based on (i) precipipation of proteins from wheat seedlings with trichloroacetic acid and acetone, (ii) solubilization of the proteins with a solution containing urea, potassium carbonate and sodium dodecyl sulfate, (iii) isoelectric focusing in an optimized pH gradient, obtained with a mixture of carrier ampholytes (Pharmalyte and Servalyt), and (iv) running elecrophoresis in the second dimension on gels with increased surface.