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Elimination of point streaking on silver stained two-dimensional gels by addition of iodoacetamide to the equilibration buffer

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Abstract

Silver staining artifacts, unrelated to protein, such as horizontal lines and vertical micro or point streaking on two-dimensional gels with carrier ampholytes or immobilized pH gradients in the first dimension are efficiently eliminated by adding iodoacetamide to the equilibration buffer of the first-dimensional gel.

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