Reduced area two-dimensional gels for direct digital imaging of radioactive protein profiles

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Abstract

A method is demonstrated which reduces the area of a standard two-dimensional polyacrylamide electrophoresis gel to approximately one-fourth of its original area. Microdensitometry is used to show that the technique did not significantly alter the reproducibility of the gels. This reduction in size makes it possible to use a micro-channel plate analyzer to directly digitize an entire gel.

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