Dodecyl maltoside detergent improves resolution of hepatic membrane proteins in two-dimensional gels

Authors

  • Dr. Frank Witzmann,

    Corresponding author
    1. Molecular Anatomy Laboratory, Indiana University-Purdue University at Indianapolis, Columbus Campus, Columbus, IN
    • Molecular Anatomy Laboratory, Department of Biology, IUPUI-Columbus, 4601 Central Avenue, Columbus, IN 47203, USA
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  • Bruce Jarnot,

    1. Toxic Hazard Division, Detachment 1, Armstrong Laboratory, Wright-Patterson AFB, OH
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  • Dona Parker

    1. Molecular Anatomy Laboratory, Indiana University-Purdue University at Indianapolis, Columbus Campus, Columbus, IN
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Abstract

This report describes the incorporation of an alkyl maltoside detergent in two-dimensional polyacrylamide gel Electrophoresis (2-D PAGE) sample lysis buffer in order to improve resolution of protein patterns separated by nonequilibrium pH gradient Electrophoresis. Membrane-associated proteins with alkaline isoelectric points form horizontal streaks on two-dimensional electrophoretograms when solubilized with conventional nonionic detergent. Dodecyl maltoside enhances protein delipidation during solubilization and improves pattern resolution and protein mobility.

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