1522-2683/asset/olbannerleft.jpg?v=1&s=212fcff8f34cb7f7728bda27b64237d82f5bc5d4)
Article
Two-dimensional electrophoresis of the total polypeptides in ripe red grape berries
Article first published online: 13 APR 2005
DOI: 10.1002/elps.1150130118
Copyright © 1992 VCH Verlagsgesellschaft mbH
1522-2683/asset/cover.gif?v=1&s=a46778197acda8c447d4e2dcfc227b0d99b34430 "ELECTROPHORESIS")
Additional Information
How to Cite
Tesnière, C. and Robin, J.-P. (1992), Two-dimensional electrophoresis of the total polypeptides in ripe red grape berries. ELECTROPHORESIS, 13: 93–96. doi: 10.1002/elps.1150130118
Publication History
- Issue published online: 13 APR 2005
- Article first published online: 13 APR 2005
- Manuscript Revised: 3 SEP 1991
- Manuscript Received: 3 APR 1991
- Abstract
- References
- Cited By
Abstract
The total polypetide composition of mature grape berries was analyzed by one-dimensional sodium dodecyl sulfate - polyacrylamide gel electrophoresis and two-dimensional electrophoresis (isoelectric focusing in the first dimension followed by sodium dodecyl sulfate - polyacrylamide gel electrophoresis in the second dimension), followed by Coomassie Blue and nitrate silver staining, respectively. Adapted methods for total protein preparation of grapes and for two-dimensional gel electrophoretic separation of polypeptides are presented. The grape patterns presented up to 52 fractions with Mrs ranging from 15000 to 110000. The polypeptides displayed pIs from 4.6 to 7.3. A group of spots from Mr 28000 to 83 000 and with a pI from 4.6 to 5.4 was strongly silver stained. The Mr 28 000 spot, pI 4.6, was revealed to be a complex of four fractions. Reproducible separations were obtained with the different carrier ampholyte mixtures tested.