Article

Monitoring by two-dimensional electrophoresis somatic embryogenesis in leaf and petiole explants from Vitis

  1. Dr. Elisabetta Gianazza1,*,
  2. Paola De Ponti1,
  3. Attilio Scienza2,3,
  4. Pierluigi Villa2,
  5. Lucia Martinelli3

Article first published online: 13 APR 2005

DOI: 10.1002/elps.1150130142

Issue

ELECTROPHORESIS

ELECTROPHORESIS

Volume 13, Issue 1, pages 203–209, 1992

Additional Information

How to Cite

Gianazza, E., De Ponti, P., Scienza, A., Villa, P. and Martinelli, L. (1992), Monitoring by two-dimensional electrophoresis somatic embryogenesis in leaf and petiole explants from Vitis. ELECTROPHORESIS, 13: 203–209. doi: 10.1002/elps.1150130142

Author Information

  1. 1

    Dipartimento di Scienze e Tecnologie Biomediche, Università di Milano

  2. 2

    Istituto di Coltivazioni Arboree, Università di Milano

  3. 3

    Laboratorio di Biotecnologie, Istituto Agrario San Michele

*Istituto di Scienze Farmacologiche, Facoltà di Farmacia, Via Balzaretti 9, I-20133 Milano, Italy

Publication History

  1. Issue published online: 13 APR 2005
  2. Article first published online: 13 APR 2005
  3. Manuscript Received: 28 JUN 1991

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Abstract

A two-dimensional electrophoretic analysis of the total proteins was carried out in Vitis rupestris as model system in order to characterize the different developmental stages – from callus to plantlets – of somatic embryogenesis events in the grapevine. The patterns of callus, embryogenetic callus, somatic embryos and plantlets derived from leaf and petiole explants were compared. Each differentiation step was characterized by specific peptide spots.

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