Nucleic acid

Validation of highly discriminating multiplex short tandem repeat amplification systems for individual identification

  1. Dr. Colin P. Kimpton*,
  2. Nicola J. Oldroyd,
  3. Stephanie K. Watson,
  4. Rachael R. E. Frazier,
  5. Peter E. Johnson,
  6. Emma S. Millican,
  7. Andrew Urquhart,
  8. Becky L. Sparkes,
  9. Peter Gill

Article first published online: 14 APR 2005

DOI: 10.1002/elps.1150170802

Issue

ELECTROPHORESIS

ELECTROPHORESIS

Volume 17, Issue 8, pages 1283–1293, 1996

Additional Information

How to Cite

Kimpton, C. P., Oldroyd, N. J., Watson, S. K., Frazier, R. R. E., Johnson, P. E., Millican, E. S., Urquhart, A., Sparkes, B. L. and Gill, P. (1996), Validation of highly discriminating multiplex short tandem repeat amplification systems for individual identification. ELECTROPHORESIS, 17: 1283–1293. doi: 10.1002/elps.1150170802

Author Information

  1. Forensic Science Service, Priory House, Birmingham, UK

*Forensic Science Service, Priory House, Gooch Street North, Birmingham B5 6QQ. UK (Tel: +121–607–6874; Fax: +121–622–2051)

Publication History

  1. Issue published online: 14 APR 2005
  2. Article first published online: 14 APR 2005
  3. Manuscript Received: 17 MAR 1996

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Keywords:

  • Individual identification;
  • Short tandem repeats;
  • Multiplex;
  • Forensics

Abstract

Short tandem repeat (STR) loci are routinely employed for individual identification. We have examined the performance and reproductibility of a highly informative co-amplification system containing the tetranucleotide STR loci: HUMVWFA31/A, HUMTH01, D20S85, D8S1179, HUMFIBRA, D21S11, and D18S51, in conjunction with the amelogenin sex test, in addition to a modified system omitting the locus D20S85. Polymerase chain reaction (PCR) products were fluorescently detected on an automated sequencer and automatically sized against an internal size standard by Genescan software. Both systems were routinely able to type 500 pg of undegraded DNA. At DNA concentrations between 50–500 pg, partial profiles were produced, but no allelic drop-out was observed. Balanced amplification of all loci occurred over a wide range of DNA concentrations from 50 pg to 10 ng. Alteration of reagent concentrations and cycling parameters from optimal resulted in variation in the efficiency of individual locus amplification relative to the other loci within the system. This was also observed at high ionic strength or extreme pH. However, at all reagent concentrations and conditions, allelic drop-out was not observed. These multiplex systems have potential in both routine forensic and intelligence database applications.

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