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Research Article
Search for serum protein-binding disaccharides and disaccharide-binding serum proteins by affinity capillary electrophoresis
Article first published online: 1 MAR 2004
DOI: 10.1002/elps.200305765
Copyright © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
1522-2683/asset/cover.gif?v=1&s=a46778197acda8c447d4e2dcfc227b0d99b34430 "ELECTROPHORESIS")
Additional Information
How to Cite
Taga, A., Yamamoto, Y., Maruyama, R. and Honda, S. (2004), Search for serum protein-binding disaccharides and disaccharide-binding serum proteins by affinity capillary electrophoresis. ELECTROPHORESIS, 25: 876–881. doi: 10.1002/elps.200305765
Publication History
- Issue published online: 1 MAR 2004
- Article first published online: 1 MAR 2004
- Manuscript Received: 30 JUN 2003
- Abstract
- References
- Cited By
Keywords:
- Affinity capillary electrophoresis;
- Disaccharide-binding human serum proteins;
- Human serum protein-binding disaccharides;
- Microscale search
Abstract
The potential use of affinity capillary electrophoresis in a microscale search for mutually interacting substances in biological fluid is demonstrated. Some disaccharides, especially gentiobiose (Gen), derivatized with 1-phenyl-3-methyl-5-pyrazolone, caused peak retardation when electrophoresed in a neutral running buffer, containing human serum. Gen, the most significantly retarded disaccharide, was converted to its negatively charged bis-mercaptoethanesulfonate derivative (MerESGen), and a serum sample was analyzed in a neutral buffer containing the derivatized disaccharide. Two peaks, belonging to the β-globulin fraction, were found to be remarkably retarded in the buffer containing MerES-Gen in a concentration-dependent way. These findings prove an interaction between disaccharides and serum proteins.