Research Article

Distribution of single DNA molecule electrophoretic mobilities in semidilute and dilute hydroxyethylcellulose solutions

  1. Yoshinori Yamaguchi1,
  2. Todor I. Todorov1,
  3. Michael D. Morris1,*,
  4. Ronald G. Larson2

Article first published online: 13 APR 2004

DOI: 10.1002/elps.200305783

Issue

ELECTROPHORESIS

ELECTROPHORESIS

Volume 25, Issue 7-8, pages 999–1006, No. 7-8 April 2004

Additional Information

How to Cite

Yamaguchi, Y., Todorov, T. I., Morris, M. D. and Larson, R. G. (2004), Distribution of single DNA molecule electrophoretic mobilities in semidilute and dilute hydroxyethylcellulose solutions. ELECTROPHORESIS, 25: 999–1006. doi: 10.1002/elps.200305783

Author Information

  1. 1

    Department of Chemistry

  2. 2

    Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA

*Department of Chemistry, University of Michigan, Ann Arbor, MI 48109–1055, USA Fax: +734-615-3790

Publication History

  1. Issue published online: 13 APR 2004
  2. Article first published online: 13 APR 2004
  3. Manuscript Received: 3 OCT 2003

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (123K)

Keywords:

  • Capillary electrophoresis;
  • DNA;
  • Electrophoretic mobility;
  • Polymer solution;
  • Rectangular capillary;
  • Video microscopy

Abstract

The distribution of center of mass electrophoretic mobility mobilities and normalized migration time of up to 1080 λ DNA molecules per experiment were measured in both semidilute hydroxyethylcellulose HEC/0.5× Tris-borate-EDTA (TBE) solutions and dilute HEC/0.5×TBE solution by high-speed video microscopy. Measurements were made microscopically over a short migration distance in homogeneous DNA HEC/0.5×TBE solution and after electrophoretic migration of a plug of DNA through 7 cm. Video at 120 frames/s (semidilute HEC solution) and 236 frames/s (dilute HEC solution) allowed visualization with adequate resolution for single molecule mobility measurements. The electrophoretic migration times and band shapes predicted from the measurements corresponded well with those measured by conventional capillary electrophoresis (CE) in both semidilute and dilute HEC. In semidilute solution, the band width predicted by a square root of time scaling is in good agreement with the results of conventional CE. However, in dilute solution the precision of the measurements was not good enough to allow scaled estimates of band widths.

Get PDF (123K)